VierheiligKnoblauchJuergensenEtAl2001

Référence

Vierheilig, H., Knoblauch, M., Juergensen, K., van Bel, A.J.E., Grundler, F.M.W. and Piche, Y. (2001) Imaging arbuscular mycorrhizal structures in living roots of Nicotiana tabacum by light, epifluorescence, and confocal laser scanning microscopy. Canadian Journal of Botany, 79(2):231-237.

Résumé

Light and epifluorescence (blue light excitation) microscopy was used to obtain micrographs of the same sections of unstained (living roots) and stained (dead) tobacco (Nicotiana tabacum L.) roots colonized by the arbuscular mycorrhizal fungus Glomus mosseae (Nicol. & Gerd.) Gerd. & Trappe. To visualize all mycorrhizal structures, roots were in situ stained with trypan blue. The metabolically active fungal tissue was determined by an in situ succinate dehydrogenase stain. A comparison of micrographs of unstained and stained mycorrhizal tobacco roots revealed that (i) finely branched arbuscules do not autofluoresce, but high autofluorescence was observed in clumped structures of collapsed arbuscules; and (ii) finely branched arbuscules are metabolically active, but no activity can be detected in autofluorescent collapsed arbuscules. Confocal laser scanning microscopy was used in combination with the two fluorochromes 5(6)-carboxyfluorescein diacetate or 5(6)-carboxy-seminaphthorhodafluor. Both fluorochromes administered to abraded tobacco leaves are transported via the phloem to the roots. Loading plants with 5(6)-carboxyfluorescein diacetate resulted in a fluorescence of root cells with highly branched arbuscules. After loading the phloem with 5(6)-carboxy-seminaphthorhodafluor, all fungal structures in the root (from relatively thick hyphae to finest branches of arbuscules) were clearly visible in the intact root. The transport route of compounds from the plants to arbuscular mycorrhizal fungi is discussed.

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@ARTICLE { VierheiligKnoblauchJuergensenEtAl2001,
    AUTHOR = { Vierheilig, H. and Knoblauch, M. and Juergensen, K. and van Bel, A.J.E. and Grundler, F.M.W. and Piche, Y. },
    TITLE = { Imaging arbuscular mycorrhizal structures in living roots of Nicotiana tabacum by light, epifluorescence, and confocal laser scanning microscopy },
    JOURNAL = { Canadian Journal of Botany },
    YEAR = { 2001 },
    VOLUME = { 79 },
    PAGES = { 231-237 },
    NUMBER = { 2 },
    NOTE = { Times Cited: 9 Article English Cited References Count: 46 412yh },
    ABSTRACT = { Light and epifluorescence (blue light excitation) microscopy was used to obtain micrographs of the same sections of unstained (living roots) and stained (dead) tobacco (Nicotiana tabacum L.) roots colonized by the arbuscular mycorrhizal fungus Glomus mosseae (Nicol. & Gerd.) Gerd. & Trappe. To visualize all mycorrhizal structures, roots were in situ stained with trypan blue. The metabolically active fungal tissue was determined by an in situ succinate dehydrogenase stain. A comparison of micrographs of unstained and stained mycorrhizal tobacco roots revealed that (i) finely branched arbuscules do not autofluoresce, but high autofluorescence was observed in clumped structures of collapsed arbuscules; and (ii) finely branched arbuscules are metabolically active, but no activity can be detected in autofluorescent collapsed arbuscules. Confocal laser scanning microscopy was used in combination with the two fluorochromes 5(6)-carboxyfluorescein diacetate or 5(6)-carboxy-seminaphthorhodafluor. Both fluorochromes administered to abraded tobacco leaves are transported via the phloem to the roots. Loading plants with 5(6)-carboxyfluorescein diacetate resulted in a fluorescence of root cells with highly branched arbuscules. After loading the phloem with 5(6)-carboxy-seminaphthorhodafluor, all fungal structures in the root (from relatively thick hyphae to finest branches of arbuscules) were clearly visible in the intact root. The transport route of compounds from the plants to arbuscular mycorrhizal fungi is discussed. },
    KEYWORDS = { glomales mycorrhiza fluorescence sdh confocal transport fungus glomus-mosseae arabidopsis-thaliana heterodera-schachtii plant infection phloem autofluorescence transport visualization accumulation },
    OWNER = { brugerolles },
    TIMESTAMP = { 2007.12.05 },
}

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