StefaniTanguayPelletierEtAl2010

Référence

Stefani, F.O.P., Tanguay, P., Pelletier, G., Piche, Y. and Hamelin, R.C. (2010) Impact of Endochitinase-Transformed White Spruce on Soil Fungal Biomass and Ectendomycorrhizal Symbiosis. Applied and Environmental Microbiology, 76(8):2607-2614. (Scopus )

Résumé

The impact of transgenic white spruce [Picea glauca (Moench) Voss] containing the endochitinase gene (ech42) on soil fungal biomass and on the ectendomycorrhizal fungi Wilcoxina spp. was tested using a greenhouse trial. The measured level of endochitinase in roots of transgenic white spruce was up to 10 times higher than that in roots of nontransformed white spruce. The level of endochitinase in root exudates of three of four ech42-transformed lines was significantly greater than that in controls. Analysis soil ergosterol showed that the amount of fungal biomass in soil samples from control white spruce was slightly larger than that in soil samples from ech42-transformed white spruce. Nevertheless, the difference was not statistically significant. The rates of mycorrhizal colonization of transformed lines and controls were similar. Sequencing the internal transcribed spacer rRNA region revealed that the root tips were colonized by the ectendomycorrhizal fungi Wilcoxina spp. and the dark septate endophyte Phialocephala fortinii. Colonization of root tips by Wilcoxina spp. was monitored by real-time PCR to quantify the fungus present during the development of ectendomycorrhizal symbiosis in ech42-transformed and control lines. The numbers of Wilcoxina molecules in the transformed lines and the controls were not significantly different (P > 0.05, as determined by analysis of covariance), indicating that in spite of higher levels of endochitinase expression, mycorrhization was not inhibited. Our results indicate that the higher levels of chitinolytic activity in root exudates and root tissues from ech42-transformed lines did not alter the soil fungal biomass or the development of ectendomycorrhizal symbiosis Involving Wilcoxina spp. Copyright © 2010, American Society for Microbiology. All Rights Reserved.

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@ARTICLE { StefaniTanguayPelletierEtAl2010,
    AUTHOR = { Stefani, F.O.P. and Tanguay, P. and Pelletier, G. and Piche, Y. and Hamelin, R.C. },
    TITLE = { Impact of Endochitinase-Transformed White Spruce on Soil Fungal Biomass and Ectendomycorrhizal Symbiosis },
    JOURNAL = { Applied and Environmental Microbiology },
    YEAR = { 2010 },
    VOLUME = { 76 },
    PAGES = { 2607-2614 },
    NUMBER = { 8 },
    ABSTRACT = { The impact of transgenic white spruce [Picea glauca (Moench) Voss] containing the endochitinase gene (ech42) on soil fungal biomass and on the ectendomycorrhizal fungi Wilcoxina spp. was tested using a greenhouse trial. The measured level of endochitinase in roots of transgenic white spruce was up to 10 times higher than that in roots of nontransformed white spruce. The level of endochitinase in root exudates of three of four ech42-transformed lines was significantly greater than that in controls. Analysis soil ergosterol showed that the amount of fungal biomass in soil samples from control white spruce was slightly larger than that in soil samples from ech42-transformed white spruce. Nevertheless, the difference was not statistically significant. The rates of mycorrhizal colonization of transformed lines and controls were similar. Sequencing the internal transcribed spacer rRNA region revealed that the root tips were colonized by the ectendomycorrhizal fungi Wilcoxina spp. and the dark septate endophyte Phialocephala fortinii. Colonization of root tips by Wilcoxina spp. was monitored by real-time PCR to quantify the fungus present during the development of ectendomycorrhizal symbiosis in ech42-transformed and control lines. The numbers of Wilcoxina molecules in the transformed lines and the controls were not significantly different (P > 0.05, as determined by analysis of covariance), indicating that in spite of higher levels of endochitinase expression, mycorrhization was not inhibited. Our results indicate that the higher levels of chitinolytic activity in root exudates and root tissues from ech42-transformed lines did not alter the soil fungal biomass or the development of ectendomycorrhizal symbiosis Involving Wilcoxina spp. Copyright © 2010, American Society for Microbiology. All Rights Reserved. },
    ADDRESS = { Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy, Québec, QC GIV 4C7, Canada },
    COMMENT = { Export Date: 11 June 2010 Source: Scopus CODEN: AEMID doi: 10.1128/AEM.02807-09 },
    ISSN = { 00992240 (ISSN) },
    KEYWORDS = { Analysis of covariances, Chitinolytic activity, Control lines, Endochitinase, Fungal biomass, In-control, Internal transcribed spacers, Mycorrhizal colonization, Mycorrhization, Picea glauca, Real-time PCR, Root exudates, Root tip, Root tissues, Soil sample, Transgenics, White spruce, Biomass, Fungi, Regression analysis, RNA, Soil surveys, Soils, Forestry, colonization, coniferous tree, endophyte, exudation, mycorrhiza, phytomass, polymerase chain reaction, real time, soil microorganism, symbiosis, transgenic plant },
    OWNER = { Luc },
    TIMESTAMP = { 2010.06.11 },
    URL = { http://www.scopus.com/inward/record.url?eid=2-s2.0-77950581747&partnerID=40&md5=ff9f184af987dee514c1b9bb5619412e },
}

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