RousseauBenhamouChetEtAl1996

Référence

Rousseau, A., Benhamou, N., Chet, I. and Piche, Y. (1996) Mycoparasitism of the extramatrical phase of Glomus intraradices by Trichoderma harzianum. Phytopathology, 86(5):434-443.

Résumé

In the present study, the interaction between the saprophytic fungus Trichoderma harzianum and the arbuscular mycorrhizal (AM) fungus Glomus intraradices was studied by transmission electron microscopy (TEM) to delineate precisely the relationship established between both partners. An axenic system, divided into four compartments, proved useful for studying the interaction between T. harzianum and the extramatrical phase of G. intraradices. This experimental model, based on root-organ culture to obtain typical mycorrhizal infections, was selected as a reliable means of obtaining mycorrhizal spores and mycelium in root-free compartments. TEM observations of samples from the interaction region showed that hyphae of T. harzianum proliferated abundantly at the spore surface and penetrated the thick host wall through local hydrolysis of the wall polymers. Hyphae of the antagonist also were seen in the subtending hyphae of the AM fungus, and they grew actively in the main host hyphae. This massive colonization was associated with marked cell damage, involving partial to complete disorganization of the cytoplasm, which led in most cases to loss of the protoplasm and apparent bursting of the main hyphae of G. intraradices, resulting in the release of the actively proliferating Trichoderma hyphae. At an advanced stage of the colonization process, the main hyphae of G. intraradices were perforated in many places. The use of wheat germ agglutinin/ovomucoid-gold complex for the localization of chitin monomers resulted in regular labeling of the host cell walls even when spores, subtending hyphae, and main hyphae of G. intraradices were colonized massively. Chitinolytic degradation was seen only in areas adjacent to the sites of Trichoderma penetration. According to our observations, the interaction between T. harzianum and G. intraradices involves the following events: (i) recognition and local penetration of the antagonist into mycorrhizal spores; (ii) active proliferation of antagonist cells in mycorrhizal hyphae; and (iii) release of the antagonist through moribund hyphal cells.

Format EndNote

Vous pouvez importer cette référence dans EndNote.

Format BibTeX-CSV

Vous pouvez importer cette référence en format BibTeX-CSV.

Format BibTeX

Vous pouvez copier l'entrée BibTeX de cette référence ci-bas, ou l'importer directement dans un logiciel tel que JabRef .

@ARTICLE { RousseauBenhamouChetEtAl1996,
    AUTHOR = { Rousseau, A. and Benhamou, N. and Chet, I. and Piche, Y. },
    TITLE = { Mycoparasitism of the extramatrical phase of Glomus intraradices by Trichoderma harzianum },
    JOURNAL = { Phytopathology },
    YEAR = { 1996 },
    VOLUME = { 86 },
    PAGES = { 434-443 },
    NUMBER = { 5 },
    NOTE = { Times Cited: 19 Article English Cited References Count: 58 Uj483 },
    ABSTRACT = { In the present study, the interaction between the saprophytic fungus Trichoderma harzianum and the arbuscular mycorrhizal (AM) fungus Glomus intraradices was studied by transmission electron microscopy (TEM) to delineate precisely the relationship established between both partners. An axenic system, divided into four compartments, proved useful for studying the interaction between T. harzianum and the extramatrical phase of G. intraradices. This experimental model, based on root-organ culture to obtain typical mycorrhizal infections, was selected as a reliable means of obtaining mycorrhizal spores and mycelium in root-free compartments. TEM observations of samples from the interaction region showed that hyphae of T. harzianum proliferated abundantly at the spore surface and penetrated the thick host wall through local hydrolysis of the wall polymers. Hyphae of the antagonist also were seen in the subtending hyphae of the AM fungus, and they grew actively in the main host hyphae. This massive colonization was associated with marked cell damage, involving partial to complete disorganization of the cytoplasm, which led in most cases to loss of the protoplasm and apparent bursting of the main hyphae of G. intraradices, resulting in the release of the actively proliferating Trichoderma hyphae. At an advanced stage of the colonization process, the main hyphae of G. intraradices were perforated in many places. The use of wheat germ agglutinin/ovomucoid-gold complex for the localization of chitin monomers resulted in regular labeling of the host cell walls even when spores, subtending hyphae, and main hyphae of G. intraradices were colonized massively. Chitinolytic degradation was seen only in areas adjacent to the sites of Trichoderma penetration. According to our observations, the interaction between T. harzianum and G. intraradices involves the following events: (i) recognition and local penetration of the antagonist into mycorrhizal spores; (ii) active proliferation of antagonist cells in mycorrhizal hyphae; and (iii) release of the antagonist through moribund hyphal cells. },
    KEYWORDS = { biocontrol agent chitinase parasitism transformed roots vesicular arbuscular mycorrhiza cell-wall chitinolytic enzymes rhizoctonia-solani biological-control life-cycle fungus chitobiosidase endochitinase recognition },
    OWNER = { brugerolles },
    TIMESTAMP = { 2007.12.05 },
}

********************************************************** ***************** Facebook Twitter *********************** **********************************************************

Abonnez-vous à
l'Infolettre du CEF!

********************************************************** ***************** Pub - Mycorhizes_2019 ****************** **********************************************************

********************************************************** ***************** Pub - Symphonies_Boreales ****************** **********************************************************

********************************************************** ***************** Boîte à trucs *************** **********************************************************

CEF-Référence
La référence vedette !

Jérémie Alluard (2016) Les statistiques au moments de la rédaction 

  • Ce document a pour but de guider les étudiants à intégrer de manière appropriée une analyse statistique dans leur rapport de recherche.

Voir les autres...