Mentag2003

Référence

Mentag, R. (2003) Potentiel d'utilisation de la transgénèse comme outil pour l'amélioration de la résistance aux agents pathogènes chez le peuplier. Thèse de doctorat, Université Laval.

Résumé

In North America, the economic importance of poplar and its hybrids has increased over the last three decades. Unfortunately, this fast-growing species are also known to be the host of several pathogens such as Melampsora spp., Hypoxylon mammatum, Septoria musiva, Agrobacterium tumefaciens and Xanthomonas populi . In the absence of an efficient pathogen control method, we propose the study of several transgenic approaches to prevent this problematic. The interest was particularly directed toward the use of a gene coding for D4E1, a synthetic antimicrobial peptide. A hybrid poplar was transformed via Agrobacterium tumefaciens . After molecular characterization, four transformants were selected based on significant D4E1-transcript accumulation. These transgenic lines were then tested for resistance to Agrobacterium tumefaciens, Xanthomonas populi pv. populi and Hypoxylon mammatum . One transgenic poplar line, Tr23, bearing the highest transcript accumulation for the D4E1 gene, presented a significant reduction in symptoms caused by A. tumefaciens and X. populi of 65% and 57%, respectively. However, none of the transgenic poplar lines obtained showed a significant difference in disease response to the fungal pathogen H. mammatum . Understanding of the poplar molecular response to pathogenic interactions can help in selecting efficient transgenic approaches for poplar disease control. To this end, the gene induction of PAL, PR-1, and PR-4 was investigated in three poplar genotypes after inoculation with Melampsora medusae . Poplar clones were previously identified as sensitive, moderately resistant, and resistant to this fungus. PAL and PR-4 showed clear differences in their pattern of expression following the corresponding rust-poplar genotypes. Earlier and/or sustained transcript accumulations of those genes were observed in resistant genotypes. Low differences in PR-1 transcript accumulations were found between the three poplar genotypes.

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@PHDTHESIS { Mentag2003,
    AUTHOR = { Mentag, R. },
    TITLE = { Potentiel d'utilisation de la transgénèse comme outil pour l'amélioration de la résistance aux agents pathogènes chez le peuplier },
    SCHOOL = { Université Laval },
    YEAR = { 2003 },
    NOTE = { CEFTMS, Piche, Y. and Seguin, A., Rachid Mentag. Pqdt Thèse (Ph. D.)--Université Laval, 2003. Bibliogr. Comprend du texte en anglais et en français. Thèse. Foresterie et géodésie Thèse (de doctorat). Piché, Yves, directeur de thèse Séguin, Armand, directeur de thèse },
    ABSTRACT = { In North America, the economic importance of poplar and its hybrids has increased over the last three decades. Unfortunately, this fast-growing species are also known to be the host of several pathogens such as Melampsora spp., Hypoxylon mammatum, Septoria musiva, Agrobacterium tumefaciens and Xanthomonas populi . In the absence of an efficient pathogen control method, we propose the study of several transgenic approaches to prevent this problematic. The interest was particularly directed toward the use of a gene coding for D4E1, a synthetic antimicrobial peptide. A hybrid poplar was transformed via Agrobacterium tumefaciens . After molecular characterization, four transformants were selected based on significant D4E1-transcript accumulation. These transgenic lines were then tested for resistance to Agrobacterium tumefaciens, Xanthomonas populi pv. populi and Hypoxylon mammatum . One transgenic poplar line, Tr23, bearing the highest transcript accumulation for the D4E1 gene, presented a significant reduction in symptoms caused by A. tumefaciens and X. populi of 65% and 57%, respectively. However, none of the transgenic poplar lines obtained showed a significant difference in disease response to the fungal pathogen H. mammatum . Understanding of the poplar molecular response to pathogenic interactions can help in selecting efficient transgenic approaches for poplar disease control. To this end, the gene induction of PAL, PR-1, and PR-4 was investigated in three poplar genotypes after inoculation with Melampsora medusae . Poplar clones were previously identified as sensitive, moderately resistant, and resistant to this fungus. PAL and PR-4 showed clear differences in their pattern of expression following the corresponding rust-poplar genotypes. Earlier and/or sustained transcript accumulations of those genes were observed in resistant genotypes. Low differences in PR-1 transcript accumulations were found between the three poplar genotypes. },
    KEYWORDS = { Peuplier Résistance aux maladies et fléaux Aspect génétique Peuplier Amélioration Transgenèse },
    OWNER = { brugerolles },
    TIMESTAMP = { 2008.01.16 },
}

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