DiopBecardPiche1992

Référence

Diop, T.A., Becard, G. and Piche, Y. (1992) Long-Term Invitro Culture of an Endomycorrhizal Fungus, Gigaspora-Margarita, on Ri T-DNA Transformed Roots of Carrot. Symbiosis, 12(3):249-259.

Résumé

The vesicular-arbuscular mycorrhizal fungus Gigaspora margarita was inoculated on Ri T-DNA transformed roots of carrot. Both organisms were grown for a long period (one year) and various features of the symbiosis were studied quantitatively and correlated to evaluate the in vitro system. Root biomass, intramatrical mycelium and production of spores were quantified. The infection units (penetration points with typical arbuscules) simultaneously increased in number and length over the culture period, and mostly when root growth declined. Sporulation of Gigaspora margarita took place after establishment of extensive hyphal biomass (1mo) and was still actively occurring after one year in culture. The new spores (450/dish) were of the expected color, shape and size, and germinated (95%) on fresh medium without pretreatment.

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@ARTICLE { DiopBecardPiche1992,
    AUTHOR = { Diop, T.A. and Becard, G. and Piche, Y. },
    TITLE = { Long-Term Invitro Culture of an Endomycorrhizal Fungus, Gigaspora-Margarita, on Ri T-DNA Transformed Roots of Carrot },
    JOURNAL = { Symbiosis },
    YEAR = { 1992 },
    VOLUME = { 12 },
    PAGES = { 249-259 },
    NUMBER = { 3 },
    NOTE = { Times Cited: 31 Article English Cited References Count: 17 Jx031 },
    ABSTRACT = { The vesicular-arbuscular mycorrhizal fungus Gigaspora margarita was inoculated on Ri T-DNA transformed roots of carrot. Both organisms were grown for a long period (one year) and various features of the symbiosis were studied quantitatively and correlated to evaluate the in vitro system. Root biomass, intramatrical mycelium and production of spores were quantified. The infection units (penetration points with typical arbuscules) simultaneously increased in number and length over the culture period, and mostly when root growth declined. Sporulation of Gigaspora margarita took place after establishment of extensive hyphal biomass (1mo) and was still actively occurring after one year in culture. The new spores (450/dish) were of the expected color, shape and size, and germinated (95%) on fresh medium without pretreatment. },
    KEYWORDS = { gigaspora-margarita ri t-DNA transformed root invitro sporulation vesicular-arbuscular mycorrhizae sporulation viability },
    OWNER = { brugerolles },
    TIMESTAMP = { 2007.12.05 },
}

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