BagoBentivengaBrenacEtAl1998

Référence

Bago, B., Bentivenga, S.P., Brenac, V., Dodd, J.C., Piche, Y. and Simon, L. (1998) Molecular analysis of Gigaspora (Glomales, gigasporaceae). New Phytologist, 139(3):581-588.

Résumé

This work presents a cooperative effort to integrate new molecular (isozyme and SSU analyses) characters into the morphological taxonomy of the genus Gigaspora (Glomales). Previous analyses of published Gigaspora SSU sequences indicated the presence of a few polymorphic nucleotides in the region delimited by primers NS71-SSU 1492'. In our study, the SSU of 24 isolates of arbuscular mycorrhizal (AM) fungi from the Gigasporaceae were amplified and the NS71-SSU 1492' region was directly sequenced. The corresponding sequences of four more isolates of AM fungi from Gigasporaceae, already published, were also included in our analyses. Three Gigaspora groups were identified on the basis of a 6 nucleotide-long 'molecular signature': Gigaspora rosea group (G. rosea+G. albida), Gigaspora margarita group (G. margarita+G. decipiens) and Gigaspora gigantea, which constituted a group by itself. The isozyme profiles (malate dehydrogenase, MDH) of 12 of these 28 isolates, and seven other isolates not sequenced, were compared. The results obtained further supported the grouping of isolates provided by the SSU analysis. Both SSU and MDH analysis indicated that two out of the 35 isolates had been misidentified, which was confirmed when their morphology was reassessed. The use of the Gigaspora intrageneric molecular signature as a quick, unambiguous and objective method to recognize Gigaspora isolates under any (field or laboratory) experimental conditions is suggested.

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@ARTICLE { BagoBentivengaBrenacEtAl1998,
    AUTHOR = { Bago, B. and Bentivenga, S.P. and Brenac, V. and Dodd, J.C. and Piche, Y. and Simon, L. },
    TITLE = { Molecular analysis of Gigaspora (Glomales, gigasporaceae) },
    JOURNAL = { New Phytologist },
    YEAR = { 1998 },
    VOLUME = { 139 },
    PAGES = { 581-588 },
    NUMBER = { 3 },
    NOTE = { Times Cited: 27 Article English Cited References Count: 33 107vb },
    ABSTRACT = { This work presents a cooperative effort to integrate new molecular (isozyme and SSU analyses) characters into the morphological taxonomy of the genus Gigaspora (Glomales). Previous analyses of published Gigaspora SSU sequences indicated the presence of a few polymorphic nucleotides in the region delimited by primers NS71-SSU 1492'. In our study, the SSU of 24 isolates of arbuscular mycorrhizal (AM) fungi from the Gigasporaceae were amplified and the NS71-SSU 1492' region was directly sequenced. The corresponding sequences of four more isolates of AM fungi from Gigasporaceae, already published, were also included in our analyses. Three Gigaspora groups were identified on the basis of a 6 nucleotide-long 'molecular signature': Gigaspora rosea group (G. rosea+G. albida), Gigaspora margarita group (G. margarita+G. decipiens) and Gigaspora gigantea, which constituted a group by itself. The isozyme profiles (malate dehydrogenase, MDH) of 12 of these 28 isolates, and seven other isolates not sequenced, were compared. The results obtained further supported the grouping of isolates provided by the SSU analysis. Both SSU and MDH analysis indicated that two out of the 35 isolates had been misidentified, which was confirmed when their morphology was reassessed. The use of the Gigaspora intrageneric molecular signature as a quick, unambiguous and objective method to recognize Gigaspora isolates under any (field or laboratory) experimental conditions is suggested. },
    KEYWORDS = { molecular analysis ssu sequences arbuscular mycorrhizal fungi gigaspora malate dehydrogenase isozyme profiles arbuscular mycorrhizal fungi endomycorrhizal fungi glomus-mosseae morphological characters colonizing roots identification diversity pcr amplification primers },
    OWNER = { brugerolles },
    TIMESTAMP = { 2007.12.05 },
}

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