RahmanDayanandanRajora2000

Référence

Rahman, M.H., Dayanandan, S. and Rajora, O.P. (2000) Microsatellite DNA markers in Populus tremuloides. Genome, 43(2):293-297.

Résumé

Markers for eight new microsatellite DNA or simple sequence repeat (SSR) loci were developed and characterized in trembling aspen (Populus tremuloides)-from a partial genomic library. Informativeness of these microsatellite DNA markers was examined by determining polymorphisms in 38 P. tremuloides individuals. Inheritance of selected markers was tested in progenies of controlled crosses. Six characterized SSR loci were of dinucleotide repeats (two perfect and four imperfect), and one each of trinucleotide and tetranucleotide repeats. The monomorphic SSR locus (PTR15) was of a compound imperfect dinucleotide repeat. The primers of one highly polymorphic SSR locus (PTR7) amplified two loci, and alleles could not be assigned to a specific locus. At the other six polymorphic loci, 25 alleles were detected in 38 P tremuloides individuals; the number of alleles ranged from 2 to 7, with an average of 4.2 alleles per locus, and the observed heterozygosity ranged from 0.05 to 0.61, with an average of 0.36 per locus. The two perfect dinucleotide and one trinucleotide microsatellite DNA loci were the most informative. Microsatellite DNA variants of four SSR loci characterized previously followed a single-locus Mendelian inheritance pattern, whereas those of PTR7 from the present study showed a two-locus Mendelian inheritance pattern in controlled crosses. The microsatellite DNA markers developed and reported here could be used for assisting various genetic, breeding, biotechnology, genome mapping, conservation, and sustainable forest management programs in poplars.

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@ARTICLE { RahmanDayanandanRajora2000,
    AUTHOR = { Rahman, M.H. and Dayanandan, S. and Rajora, O.P. },
    TITLE = { Microsatellite DNA markers in Populus tremuloides },
    JOURNAL = { Genome },
    YEAR = { 2000 },
    VOLUME = { 43 },
    PAGES = { 293-297 },
    NUMBER = { 2 },
    NOTE = { 08312796 (ISSN) Cited By (since 1996): 16 Export Date: 27 April 2007 Source: Scopus CODEN: GENOE Language of Original Document: English Correspondence Address: Rajora, O.P.; Dept. of Biology; Dalhousie University Halifax, NS B3H 4J1, Canada; email: Om.Rajora@Dal.ca Molecular Sequence Numbers: GENBANK: AF240755, AF240756, AF240757, AF240758, AF240759, AF240760, AF240761, AF240762 References: Beckman, J.S., Soller, M., Toward a unified approach to the genetic mapping of eukaryotes based on sequence-tagged microsatellite sites (1990) Bio/Technology, 8, pp. 930-932; Dayanandan, S., Rajora, O.P., Bawa, K.S., Isolation and characterization of microsatellites in trembling aspen (Populus tremuloides) (1998) Theor. Appl. Genet., 96, pp. 950-956; Devey, M.E., Bell, J.C., Smith, D.N., Neale, D.B., Moran, G.F., A genetic linkage map for Pinus radiata based on RFLP, RAPD, and microsatellite markers (1996) Theor. Appl. Genet., 92, pp. 673-679; Echt, C.S., May-Marquardt, P., Hseih, M., Zahorchak, R., Characterization of microsatellite markers in eastern white pine (1996) Genome, 39, pp. 1102-1108; Fisher, P.J., Richardson, T.E., Gardner, R.C., Characteristics of single- and multi-copy microsatellites from Pinus radiata (1998) Theor. Appl. Genet., 96, pp. 969-979; Hearne, C., Ghosh, S., Todd, J., Microsatellites for linkage analysis of genetic traits (1992) Trends Genet., 8, pp. 288-294; Kostia, S., Sirkaa-Liisa, V., Vakkari, P., Pulkkinen, P., Microsatellite sequences in a conifer, Pinus sylvestris (1995) Genome, 38, pp. 1244-1248; Marques, C.M., Brondani, R.P.V., Grattapaglia, D., Sederoff, R., Conservation of microsatellite loci and QTL in Eucalyptus species (1999) Program and Abstract Guide of the Plant and Animal Genome VII Conference, , held at San Diego, Calif., 17-21 January 1999; Paglia, G.P., Olivieri, A.M., Morgante, M., Towards second-generation STS (sequence-tagged sites) linkage maps in conifers: A genetic map of Norway spruce (Picea abies K.) (1998) Mol. Gen. Genet., 258, pp. 466-478; Pfeiffer, A., Olivieri, A.M., Morgante, M., Identification and characterization of microsatellites in Norway spruce (Picea abies K) (1997) Genome, 40, pp. 411-419; Powell, W., Morgante, M., McDevitt, R., Vendramin, G.G., Rafalski, J.A., Polymorphic simple sequence repeat regions in chloroplast genomes: Application to population genetics of pines (1995) Proc. Natl. Acad. Sci. U.S.A., 92, pp. 7759-7763; Rajora, O.P., Dancik, B.P., Chloroplast DNA variation in Populus. I. Intraspecific restriction fragment diversity within Populus deltoides, P. nigra, and P. maximowiczii (1995) Theor. Appl. Genet., 90, pp. 317-323; Rajora, O.P., Rahman, M.H., Buchert, G.P., Dancik, B.P., Microsatellite DNA analysis of genetic effects of harvesting in old-growth eastern white pine (Pinus strobus) in Ontario (2000) Mol. Ecol., 9, pp. 339-348; Sanchez-Escribano, E.M., Martin, J.P., Carreno, J., Cenis, J.L., Use of sequence-tagged microsatellite site markers for characterizing table grape cultivars (1999) Genome, 42, pp. 87-93; Smith, D.N., Devey, M.E., Occurrence and inheritance of microsatellites in Pinus radiata (1994) Genome, 37, pp. 977-983; Sokal, R.R., Rohlf, F.J., (1969) Biometry, , W.H. Freeman and Co., San Francisco; Tautz, D., Hypervariability of simple sequences as a general source of polymorphic markers (1989) Nucleic Acids Res., 17, pp. 6463-6471; Van De Ven, W.T.G., McNicol, R.J., Microsatellites as DNA markers in Sitka spruce (1996) Theor. Appl. Genet., 93, pp. 613-617; Wang, Z., Weber, J.L., Zhong, G., Tanksley, S.D., Survey of plant short tandem repeats (1994) Theor. Appl. Genet., 88, pp. 1-6; Weber, J.L., May, P.E., Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction (1989) Am. J. Hum. Genet., 44, pp. 388-396; Weising, K., Winter, P., Huttel, B., Kahl, G., Microsatellite markers for molecular breeding (1998) J. Crop Prod., 1, pp. 113-143. },
    ABSTRACT = { Markers for eight new microsatellite DNA or simple sequence repeat (SSR) loci were developed and characterized in trembling aspen (Populus tremuloides)-from a partial genomic library. Informativeness of these microsatellite DNA markers was examined by determining polymorphisms in 38 P. tremuloides individuals. Inheritance of selected markers was tested in progenies of controlled crosses. Six characterized SSR loci were of dinucleotide repeats (two perfect and four imperfect), and one each of trinucleotide and tetranucleotide repeats. The monomorphic SSR locus (PTR15) was of a compound imperfect dinucleotide repeat. The primers of one highly polymorphic SSR locus (PTR7) amplified two loci, and alleles could not be assigned to a specific locus. At the other six polymorphic loci, 25 alleles were detected in 38 P tremuloides individuals; the number of alleles ranged from 2 to 7, with an average of 4.2 alleles per locus, and the observed heterozygosity ranged from 0.05 to 0.61, with an average of 0.36 per locus. The two perfect dinucleotide and one trinucleotide microsatellite DNA loci were the most informative. Microsatellite DNA variants of four SSR loci characterized previously followed a single-locus Mendelian inheritance pattern, whereas those of PTR7 from the present study showed a two-locus Mendelian inheritance pattern in controlled crosses. The microsatellite DNA markers developed and reported here could be used for assisting various genetic, breeding, biotechnology, genome mapping, conservation, and sustainable forest management programs in poplars. },
    KEYWORDS = { DNA fingerprinting Genetic mapping Microsatellites Poplar Simple sequence repeat (SSR) markers genetic marker microsatellite DNA Genes, Plant Microsatellite Repeats Minisatellite Repeats Populus tremuloides },
    OWNER = { brugerolles },
    TIMESTAMP = { 2007.12.05 },
}

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