DayanandanRajoraBawa1998

Référence

Dayanandan, S., Rajora, O.P., Bawa, K.S. (1998) Isolation and characterization of microsatellites in trembling aspen (Populus tremuloides). Theoretical and Applied Genetics, 96(6-7):950-956.

Résumé

We have identified, isolated, and characterized microsatellite/simple sequence repeat (SSR) loci in trembling aspen (Populus tremuloides) by screening partial genomic libraries. We have also examined the compatibility and use of the P. tremuloides SSR primers to resolve microsatellites in other Populus species. Fourteen microsatellites were identified from 1600 clones screened. The TC/AG microsatellites were the most abundant. A total of 29 alleles were detected in 36 P. tremuloides individuals at the four SSR loci (two each of di- and tri-nucleotide repeats) characterized. The number of alleles at the SSR loci ranged from 5 to 11, with an average of 7.25 alleles per locus, and the observed heterozygosity ranged from 0.19 to 0.82, with a mean of 0.46 per locus. Although the highest polymorphism was observed for a dinucleotide SSR locus, the trinucleotide SSR loci showed substantial polymorphism. There were 34 unique multilocus genotypes among the 36 P. tremuloides individuals examined, and 89% of the individuals had unique multilocus genotypes. Two pairs of SSR primers were successful in PCR, amplifying genomic DNA and resolving microsatellites of comparable size from Populus deltoides, P. nigra, P. x canadensis, and P. maximowiczii. The microsatellite DNA markers developed could be used for clonal fingerprinting, certification of controlled crosses, genome mapping, marker-assisted early selection, genetic diversity assessments, and conservation and sustainable management of poplar genetic resources.

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@ARTICLE { DayanandanRajoraBawa1998,
    AUTHOR = { Dayanandan, S. and Rajora, O.P. and Bawa, K.S. },
    TITLE = { Isolation and characterization of microsatellites in trembling aspen (Populus tremuloides) },
    JOURNAL = { Theoretical and Applied Genetics },
    YEAR = { 1998 },
    VOLUME = { 96 },
    PAGES = { 950-956 },
    NUMBER = { 6-7 },
    NOTE = { 00405752 (ISSN) Cited By (since 1996): 37 Export Date: 27 April 2007 Source: Scopus CODEN: THAGA doi: 10.1007/s001220050825 Language of Original Document: English Correspondence Address: Rajora, O.P.; Department of Renewable Resources; 751 General Services Building; University of Alberta Edmonton, Alta. T6G 2H1, Canada; email: om.rajora@ualberta.ca References: Barrett, J.W., Rajora, O.P., Yeh, F.C., Dancik, B.P., Strobeck, C., Mitochondrial DNA variation and genetic relationships of Populus species (1993) Genome, 36, pp. 87-93; Chase, M.R., Kesseli, R.V., Bawa, K.S., Microsatellite markers for population and conservation genetics of tropical trees (1996) Am J Bot, 83, pp. 51-57; Cheliak, W.M., Dancik, B.P., Genetic diversity of natural populations of a clone-forming tree Papulus tremuloides (1982) Can J Genet Cytol, 24, pp. 611-616; Condit, R., Hubbell, S., Abundance and DNA sequence of two-base repeat regions in tropical tree genomes (1991) Genome, 34, pp. 66-71; Dayanandan, S., Bawa, K.S., Kesseli, R.V., Conservation of microsatellites among tropical trees (Leguminosae) (1997) Am J Bot, 84 (12), pp. 1658-1663; Dewey, M.E., Bell, J.C., Smith, D.N., Neale, D.B., Moran, G.F., A genetic linkage map for Pinus radiata based on RFLP, RAPD, and microsatellite markers (1996) Theor Appl Genet, 92, pp. 673-679; Echt, C.S., Nelson, C.D., Linkage mapping and genome length in eastern white opine (Pinus strobus L) (1997) Theor Appl Genet, 94, pp. 1031-1037; Echt, C.S., May-Marquardt, P., Hseih, M., Zahorchak, R., Characterization of microsatellite markers in eastern white pine (1996) Genome, 39, pp. 1102-1108; Hearne, C., Ghosh, S., Todd, J., Microsatellites for linkage analysis of genetic traits (1992) Trends Genet, 8, pp. 288-294; Jarret, R.L., Merrick, L.C., Holms, T., Evans, J., Aradhya, M.K., Simple sequence repeats in watermelon (Citrullus lantus (Thumb.) Matsum. and Nakai) (1997) Genome, 40, pp. 433-441; Jerka, J., Pethiyagoda, C., Simple repetitive DNA sequences from primates: Compilation and analysis (1995) J Mol Evol, 40, pp. 120-126; Kijas, J.M.H., Fowler, J.C.S., Thomas, M.R., An evaluation of sequence-tagged microsatellite site markers for genetic analysis within Citrus and related species (1995) Genome, 38, pp. 349-355; Kostia, S., Sirkaa-Liisa, V., Vakkari, P., Pulkkinen, P., Microsatellite sequences in a conifer, Pinus sylvestris (1995) Genome, 38, pp. 1244-1248; Levine, I., Cheng, H.H., Bacter, J.C., Hillel, J., Turkey microsatellite DNA loci amplified by chicken specific primers (1995) Anim Genet, 26, pp. 107-110; Moore, S.S., Sargeant, L.L., King, T.J., Matick, J.S., Georges, M., Hetzel, D.J.S., The conservation of dinucleotide microsatellites among mammalian genomes allows the use of heterologous PCR primer pairs in closely related species (1991) Genomics, 10, pp. 654-660; Morgante, M., Olivieri, A.M., PCR amplified microsatellites as markers in plant genetics (1993) Plant J, 3, pp. 175-182; Ondro, W.J., Present trends and future prospects for poplar utilization in Alberta (1991) For Chron, 67, pp. 271-274; Paetkau, D., Strobeck, C., Microsatellite analysis of genetic variation in black bear populations (1994) Mol Ecol, 3, pp. 489-495; Pfeiffer, A., Olivieri, A.M., Morgante, M., Identification and characterization of microsatellites in Norway spruce (Picea ahies K) (1997) Genome, 40, pp. 411-419; Rajora, O.P., Allozymes as aids for identification and differentiation of some Populus maximowiczii Henry clonal varieties (1988) Biochem Syst Ecol, 16, pp. 635-640; Rajora, O.P., Genetic structure and identification of Populus deltoides Marsh. clones based on allozymes (1989) Genome, 32, pp. 440-448; Rajora, O.P., Characterization of 43 Populus nigra L. clones representing selections, cultivars and botanical varieties based on their multilocus allozyme genotypes (1989) Euphytica, 43, pp. 197-206; Rajora, O.P., (1991) A Long-term Program Plan for Genetic Improvement of Aspen and Other Poplar Hardwoods in Alberta, , Alberta Forest Service, Edmonton, Alberta; Rajora, O.P., Dancik, B.P., Genetic characterization and relationships of Populus alba, P. tremula and P. × canescens, and their clones (1992) Theor Appl Genet, 84, pp. 291-298; Rajora, O.P., Dancik, B.P., Chloroplast DNA inheritance in Populus (1992) Theor Appl Genet, 84, pp. 280-285; Rajora, O.P., Dancik, B.P., Chloroplast DNA variation in Populus. 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    ABSTRACT = { We have identified, isolated, and characterized microsatellite/simple sequence repeat (SSR) loci in trembling aspen (Populus tremuloides) by screening partial genomic libraries. We have also examined the compatibility and use of the P. tremuloides SSR primers to resolve microsatellites in other Populus species. Fourteen microsatellites were identified from 1600 clones screened. The TC/AG microsatellites were the most abundant. A total of 29 alleles were detected in 36 P. tremuloides individuals at the four SSR loci (two each of di- and tri-nucleotide repeats) characterized. The number of alleles at the SSR loci ranged from 5 to 11, with an average of 7.25 alleles per locus, and the observed heterozygosity ranged from 0.19 to 0.82, with a mean of 0.46 per locus. Although the highest polymorphism was observed for a dinucleotide SSR locus, the trinucleotide SSR loci showed substantial polymorphism. There were 34 unique multilocus genotypes among the 36 P. tremuloides individuals examined, and 89% of the individuals had unique multilocus genotypes. Two pairs of SSR primers were successful in PCR, amplifying genomic DNA and resolving microsatellites of comparable size from Populus deltoides, P. nigra, P. x canadensis, and P. maximowiczii. The microsatellite DNA markers developed could be used for clonal fingerprinting, certification of controlled crosses, genome mapping, marker-assisted early selection, genetic diversity assessments, and conservation and sustainable management of poplar genetic resources. },
    KEYWORDS = { Clone identification Microsatellite loci Polymorphism Poplars (Populus) Simple sequence repeats DNA isolation microsatellite DNA Populus tremuloides },
    OWNER = { brugerolles },
    TIMESTAMP = { 2007.12.05 },
}

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