SachsKembelLauEtAl2009

Reference

Sachs, J.L., Kembel, S.W., Lau, A.H., Simms, E.L. (2009) In situ phylogenetic structure and diversity of wild Bradyrhizobium communities. Applied and Environmental Microbiology, 75(14):4727-4735. (Scopus )

Abstract

Bacteria often infect their hosts from environmental sources, but little is known about how environmental and host-infecting populations are related. Here, phylogenetic clustering and diversity were investigated in a natural community of rhizobial bacteria from the genus Bradyrhizobium. These bacteria live in the soil and also form beneficial root nodule symbioses with legumes, including those in the genus Lotus. Two hundred eighty pure cultures of Bradyrhizobium bacteria were isolated and genotyped from wild hosts, including Lotus angustissimus, Lotus heermannii, Lotus micranthus, and Lotus strigosus. Bacteria were cultured directly from symbiotic nodules and from two microenvironments on the soil-root interface: root tips and mature (old) root surfaces. Bayesian phylogenies of Bradyrhizobium isolates were reconstructed using the internal transcribed spacer (ITS), and the structure of phylogenetic relatedness among bacteria was examined by host species and microenvironment. Inoculation assays were performed to confirm the nodulation status of a subset of isolates. Most recovered rhizobial genotypes were unique and found only in root surface communities, where little bacterial population genetic structure was detected among hosts. Conversely, most nodule isolates could be classified into several related, hyper-abundant genotypes that were phylogenetically clustered within host species. This pattern suggests that host infection provides ample rewards to symbiotic bacteria but that host specificity can strongly structure only a small subset of the rhizobial community. Copyright © 2009, American Society for Microbiology. All Rights Reserved.

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@ARTICLE { SachsKembelLauEtAl2009,
    AUTHOR = { Sachs, J.L. and Kembel, S.W. and Lau, A.H. and Simms, E.L. },
    TITLE = { In situ phylogenetic structure and diversity of wild Bradyrhizobium communities },
    JOURNAL = { Applied and Environmental Microbiology },
    YEAR = { 2009 },
    VOLUME = { 75 },
    PAGES = { 4727-4735 },
    NUMBER = { 14 },
    ABSTRACT = { Bacteria often infect their hosts from environmental sources, but little is known about how environmental and host-infecting populations are related. Here, phylogenetic clustering and diversity were investigated in a natural community of rhizobial bacteria from the genus Bradyrhizobium. These bacteria live in the soil and also form beneficial root nodule symbioses with legumes, including those in the genus Lotus. Two hundred eighty pure cultures of Bradyrhizobium bacteria were isolated and genotyped from wild hosts, including Lotus angustissimus, Lotus heermannii, Lotus micranthus, and Lotus strigosus. Bacteria were cultured directly from symbiotic nodules and from two microenvironments on the soil-root interface: root tips and mature (old) root surfaces. Bayesian phylogenies of Bradyrhizobium isolates were reconstructed using the internal transcribed spacer (ITS), and the structure of phylogenetic relatedness among bacteria was examined by host species and microenvironment. Inoculation assays were performed to confirm the nodulation status of a subset of isolates. Most recovered rhizobial genotypes were unique and found only in root surface communities, where little bacterial population genetic structure was detected among hosts. Conversely, most nodule isolates could be classified into several related, hyper-abundant genotypes that were phylogenetically clustered within host species. This pattern suggests that host infection provides ample rewards to symbiotic bacteria but that host specificity can strongly structure only a small subset of the rhizobial community. Copyright © 2009, American Society for Microbiology. All Rights Reserved. },
    COMMENT = { Cited By (since 1996): 10 Export Date: 17 September 2012 Source: Scopus CODEN: AEMID doi: 10.1128/AEM.00667-09 },
    ISSN = { 00992240 (ISSN) },
    KEYWORDS = { Bacterial population, Bayesian, Host-specificity, In-situ, Inoculation assay, Internal transcribed spacers, Microenvironment, Microenvironments, Nodulation, Phylogenetic structures, Pure culture, Rhizobial bacteria, Root nodules, Root surfaces, Root tip, Soil-root interface, Symbiotic bacteria, Intelligent vehicle highway systems, Soils, Bacteriology, internal transcribed spacer, Bayesian analysis, genetic structure, host-pathogen interaction, inoculation, legume, microbial community, nodulation, phylogenetics, phylogeny, rhizobacterium, species diversity, symbiosis, wild population, article, bacterial genetics, bacterium culture, bacterium isolation, Bradyrhizobium, controlled study, genetic variability, microbial community, nodulation, nonhuman, nucleotide sequence, phylogeny, Biodiversity, Bradyrhizobium, Cluster Analysis, DNA, Bacterial, DNA, Ribosomal Spacer, Lotus, Molecular Sequence Data, Phylogeny, Plant Roots, Sequence Analysis, DNA, Bacteria (microorganisms), Bradyrhizobium, Lotus angustissimus, Lotus heermannii, Lotus micranthus, Lotus strigosus },
    OWNER = { Luc },
    TIMESTAMP = { 2012.09.17 },
    URL = { http://www.scopus.com/inward/record.url?eid=2-s2.0-67650498128&partnerID=40&md5=9f298aed91fafea741538265f0acceac },
}

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