RameshaSriramaRavikanthEtAl2009

Reference

Ramesha, B.T., Srirama, R., Ravikanth, G., Ravishankar, K.V., Dayanandan, S., Sreejayan, N., Vasudeva, R., Ganeshaiah, K.N., Shaanker, R.U. (2009) Development of polymorphic microsatellite loci in Nothapodytes nimmoniana, a medicinally important tree from the Western Ghats, India. Molecular Ecology Resources, 9(1):365-367.

Abstract

Nothapodytes nimmoniana is a medicinally important tree species that occur in the Western Ghats, a megadiversity hotspot in southern India. Inner stem bark of the tree contains an important anti-cancer alkaloid, camptothecin for which the natural population of the tree is heavily harvested. In this paper, we report the isolation and characterization of eight polymorphic microsatellite loci using enrichment hybridization protocol. Analysis of 36 individuals representing two populations revealed three to 12 alleles per locus. Observed heterozygosity ranged from 0.21 to 0.94 for the two populations. None of the loci tested showed linkage disequilibrium. These markers are invaluable for evaluating the genetic structure and assessing the genetic impacts of harvesting of N. nimmoniana in the Western Ghats to formulate strategies for conservation of the species.

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@ARTICLE { RameshaSriramaRavikanthEtAl2009,
    AUTHOR = { Ramesha, B.T. and Srirama, R. and Ravikanth, G. and Ravishankar, K.V. and Dayanandan, S. and Sreejayan, N. and Vasudeva, R. and Ganeshaiah, K.N. and Shaanker, R.U. },
    TITLE = { Development of polymorphic microsatellite loci in Nothapodytes nimmoniana, a medicinally important tree from the Western Ghats, India },
    JOURNAL = { Molecular Ecology Resources },
    YEAR = { 2009 },
    VOLUME = { 9 },
    PAGES = { 365-367 },
    NUMBER = { 1 },
    MONTH = { jan },
    AF = { Ramesha, B. T.EOLEOLSrirama, R.EOLEOLRavikanth, G.EOLEOLRavishankar, K. V.EOLEOLDayanandan, S.EOLEOLSreejayan, N.EOLEOLVasudeva, R.EOLEOLGaneshaiah, K. N.EOLEOLShaanker, R. Uma },
    DE = { microsatellite; N; nimmoniana; polymorphic loci; Western Ghats; India },
    DI = { 10.1111/j.1755-0998.2008.02490.x },
    PG = { 3 },
    SN = { 1755-098X },
    UT = { ISI:000262678900092 },
    ABSTRACT = { Nothapodytes nimmoniana is a medicinally important tree species that occur in the Western Ghats, a megadiversity hotspot in southern India. Inner stem bark of the tree contains an important anti-cancer alkaloid, camptothecin for which the natural population of the tree is heavily harvested. In this paper, we report the isolation and characterization of eight polymorphic microsatellite loci using enrichment hybridization protocol. Analysis of 36 individuals representing two populations revealed three to 12 alleles per locus. Observed heterozygosity ranged from 0.21 to 0.94 for the two populations. None of the loci tested showed linkage disequilibrium. These markers are invaluable for evaluating the genetic structure and assessing the genetic impacts of harvesting of N. nimmoniana in the Western Ghats to formulate strategies for conservation of the species. },
    KEYWORDS = { CAMPTOTHECIN },
    OWNER = { brugerolles },
    TIMESTAMP = { 2009.03.13 },
}

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