AounRiouxSimardEtAl2009

Reference

Aoun, M., Rioux, D., Simard, M., Bernier, L. (2009) Fungal Colonization and Host Defense Reactions in Ulmus americana Callus Cultures Inoculated with Ophiostoma novo-ulmi. Phytopathology, 99(6):642-650.

Abstract

The host-pathogen interaction leading to Dutch elm disease was analyzed using histo- and cyto-chemical tests in an in vitro system. Friable and hard susceptible Ulmus americana callus cultures were inoculated with the highly aggressive pathogen Ophiostoma novo-ulmi. Inoculated callus tissues were compared with water-treated callus tissues and studied with light microscopy (LM), transmission-electron microscopy (TEM), and scanning-electron microscopy (SEM). New aspects of this interaction are described. These include the histological observation, for the first time in plant callus cultures, of suberin with its typical lamellar structure in TEM and the intracellular presence of O. novo-ulmi. Expression of the phenylalanine ammonia lyase gene, monitored by real-time quantitative polymerase chain reaction, was correlated with the accumulation of suberin, phenols, and lignin in infected callus cultures. This study validates the potential use of the in vitro system for genomic analyses aimed at identifying genes expressed during the interaction in the Dutch elm disease pathosystem.

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@ARTICLE { AounRiouxSimardEtAl2009,
    AUTHOR = { Aoun, M. and Rioux, D. and Simard, M. and Bernier, L. },
    TITLE = { Fungal Colonization and Host Defense Reactions in Ulmus americana Callus Cultures Inoculated with Ophiostoma novo-ulmi },
    JOURNAL = { Phytopathology },
    YEAR = { 2009 },
    VOLUME = { 99 },
    PAGES = { 642-650 },
    NUMBER = { 6 },
    MONTH = { jun },
    AF = { Aoun, MirellaEOLEOLRioux, DannyEOLEOLSimard, MarieEOLEOLBernier, Louis },
    C1 = { [Aoun, Mirella; Rioux, Danny; Simard, Marie; Bernier, Louis] Univ Laval, Ctr detude foret CEF, First & fourth authors, Quebec City, PQ G1V 0A6, Canada.EOLEOL[Aoun, Mirella; Bernier, Louis] Univ Laval, CEF, Quebec City, PQ G1V 0A6, Canada.EOLEOL[Rioux, Danny; Simard, Marie] Laurentian Forestry Ctr, Canadian Forest Serv, Nat Resources Canada, Quebec City, PQ G1V 4C7, Canada. },
    DI = { 10.1094/PHYTO-99-6-0642 },
    EM = { Louis.Bernier@sbf.ulaval.ca },
    FU = { Natural Sciences and Engineering Research Council (NSERC) of Canada },
    FX = { This work was made possible thanks to Genomics and Strategic GrantsEOLEOLfrom the Natural Sciences and Engineering Research Council (NSERC) ofEOLEOLCanada. We thank V. Jacobi for insights and for critical revision ofEOLEOLthe manuscript and A. Vialle for technical assistance. },
    GA = { 447SW },
    J9 = { PHYTOPATHOLOGY },
    JI = { Phytopathology },
    LA = { English },
    NR = { 58 },
    PA = { 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA },
    PG = { 9 },
    PI = { ST PAUL },
    RP = { Bernier, L, Univ Laval, CEF, Quebec City, PQ G1V 0A6, Canada. },
    SC = { Plant Sciences },
    SN = { 0031-949X },
    TC = { 0 },
    UT = { ISI:000266213100002 },
    ABSTRACT = { The host-pathogen interaction leading to Dutch elm disease was analyzed using histo- and cyto-chemical tests in an in vitro system. Friable and hard susceptible Ulmus americana callus cultures were inoculated with the highly aggressive pathogen Ophiostoma novo-ulmi. Inoculated callus tissues were compared with water-treated callus tissues and studied with light microscopy (LM), transmission-electron microscopy (TEM), and scanning-electron microscopy (SEM). New aspects of this interaction are described. These include the histological observation, for the first time in plant callus cultures, of suberin with its typical lamellar structure in TEM and the intracellular presence of O. novo-ulmi. Expression of the phenylalanine ammonia lyase gene, monitored by real-time quantitative polymerase chain reaction, was correlated with the accumulation of suberin, phenols, and lignin in infected callus cultures. This study validates the potential use of the in vitro system for genomic analyses aimed at identifying genes expressed during the interaction in the Dutch elm disease pathosystem. },
    KEYWORDS = { DUTCH ELM DISEASE; PHENYLALANINE AMMONIA-LYASE; INDUCED PHENYLPROPANOID METABOLISM; BARRIER ZONE FORMATION; CERATOCYSTIS-ULMI; NONHOST TREES; SUSPENSION-CULTURES; MANSONONE-F; ACCUMULATION; RESISTANT },
    OWNER = { sobru1 },
    PUBLISHER = { Amer Phytopathological Soc },
    TIMESTAMP = { 2009.06.22 },
}

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