BedonLevasseurGrima-PettenatiEtAl2009

Reference

Bedon, F., Levasseur, C., Grima-Pettenati, J., Seguin, A., MacKay, J. (2009) Sequence analysis and functional characterization of the promoter of the Picea glauca Cinnamyl Alcohol Dehydrogenase gene in transgenic white spruce plants. Plant Cell Reports, 28(5):787-800.

Abstract

The enzyme Cinnamyl Alcohol Dehydrogenase (CAD) catalyses the last step of lignin monomer synthesis, and is considered as a molecular marker of cell wall lignification in different plants species. Here, we report the isolation and analysis of 5' flanking genomic DNA regions upstream to the CAD gene, from two conifers, i.e. white spruce (Picea glauca (Moench) Voss) and loblolly pine (Pinus taeda L.). Sequence comparisons with available CAD gene promoters from angiosperms highlighted the conservation of cis-elements matching MYB, WRKY and bHLH binding sites. Functional characterization of the P. glauca CAD promoter used P. glauca seedlings stably transformed with a DNA fragment of 1,163 base pairs (PgCAD) fused to the beta-glucuronidase (GUS) gene. Histochemical observations of different vegetative organs of the transgenic trees showed that this sequence was sufficient to drive GUS expression in lignifying tissues, and more specifically in differentiating xylem cells. Quantitative RT-PCR experiments also indicated that the native CAD gene was preferentially expressed in differentiating xylem both in stems and roots. In addition, GUS expression driven by the PgCAD promoter was wound-inducible which was consistent with the accumulation of CAD mRNA in response to jasmonate application and mechanical wounding. The spruce CAD promoter represents a valuable tool for research and biotechnology applications related to xylem and wood.

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@ARTICLE { BedonLevasseurGrima-PettenatiEtAl2009,
    AUTHOR = { Bedon, F. and Levasseur, C. and Grima-Pettenati, J. and Seguin, A. and MacKay, J. },
    TITLE = { Sequence analysis and functional characterization of the promoter of the Picea glauca Cinnamyl Alcohol Dehydrogenase gene in transgenic white spruce plants },
    JOURNAL = { Plant Cell Reports },
    YEAR = { 2009 },
    VOLUME = { 28 },
    PAGES = { 787-800 },
    NUMBER = { 5 },
    MONTH = { may },
    AF = { Bedon, FrankEOLEOLLevasseur, CarolineEOLEOLGrima-Pettenati, JacquelineEOLEOLSeguin, ArmandEOLEOLMacKay, John },
    C1 = { [Bedon, Frank; MacKay, John] Univ Laval, Ctr Etud Foret, Quebec City, PQ G1V 0A6, Canada.EOLEOL[Bedon, Frank; Grima-Pettenati, Jacqueline] CNRS, UPS, UMR 5546, F-31326 Castanet Tolosan, France.EOLEOL[Levasseur, Caroline; Seguin, Armand] Laurentian Forestry Ctr, Canadian Forest Serv, Quebec City, PQ G1V 4C7, Canada. },
    DE = { Conifer; Cinnamyl alcohol dehydrogenase (CAD); Lignin; VascularEOLEOLtissues; Cis-regulatory elements; Jasmonate; Wounding },
    DI = { 10.1007/s00299-009-0688-0 },
    EM = { bedon@pierroton.inra.frEOLEOLjmackay@rsvs.ulaval.ca },
    FU = { Genome Canada ; Genome Quebec },
    FX = { We are grateful to Francoise Pelletier and Laurence Tremblay forEOLEOLexcellent assistance in tissue culture and growth of the plants inEOLEOLgreenhouse. We acknowledge Denis Lachance for helpful advice for theEOLEOLjasmonate application methods. This research was supported by fundingEOLEOLfrom Genome Canada and Genome Quebec to JM and AS for the ARBOREAEOLEOLproject. },
    GA = { 441MZ },
    J9 = { PLANT CELL REP },
    JI = { Plant Cell Reports },
    LA = { English },
    NR = { 58 },
    PA = { 233 SPRING ST, NEW YORK, NY 10013 USA },
    PG = { 14 },
    PI = { NEW YORK },
    RP = { MacKay, J, Univ Laval, Ctr Etud Foret, Quebec City, PQ G1V 0A6, Canada. },
    SC = { Plant Sciences },
    SN = { 0721-7714 },
    TC = { 0 },
    UT = { ISI:000265774600007 },
    ABSTRACT = { The enzyme Cinnamyl Alcohol Dehydrogenase (CAD) catalyses the last step of lignin monomer synthesis, and is considered as a molecular marker of cell wall lignification in different plants species. Here, we report the isolation and analysis of 5' flanking genomic DNA regions upstream to the CAD gene, from two conifers, i.e. white spruce (Picea glauca (Moench) Voss) and loblolly pine (Pinus taeda L.). Sequence comparisons with available CAD gene promoters from angiosperms highlighted the conservation of cis-elements matching MYB, WRKY and bHLH binding sites. Functional characterization of the P. glauca CAD promoter used P. glauca seedlings stably transformed with a DNA fragment of 1,163 base pairs (PgCAD) fused to the beta-glucuronidase (GUS) gene. Histochemical observations of different vegetative organs of the transgenic trees showed that this sequence was sufficient to drive GUS expression in lignifying tissues, and more specifically in differentiating xylem cells. Quantitative RT-PCR experiments also indicated that the native CAD gene was preferentially expressed in differentiating xylem both in stems and roots. In addition, GUS expression driven by the PgCAD promoter was wound-inducible which was consistent with the accumulation of CAD mRNA in response to jasmonate application and mechanical wounding. The spruce CAD promoter represents a valuable tool for research and biotechnology applications related to xylem and wood. },
    KEYWORDS = { LIGNIN BIOSYNTHETIC-PATHWAY; CELL-SPECIFIC EXPRESSION; WOOD-FORMING TISSUES; ARABIDOPSIS-THALIANA; TRANSCRIPTION FACTOR; LOBLOLLY-PINE; MOLECULAR CHARACTERIZATION; VASCULAR EXPRESSION; CIS-ELEMENTS; PROTEIN },
    OWNER = { sobru1 },
    PUBLISHER = { Springer },
    TIMESTAMP = { 2009.07.07 },
}

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