IsabelTremblayMichaudEtAl1993

Référence

Isabel, N., Tremblay, L., Michaud, M., Tremblay, F.M., Bousquet, J. (1993) Rapds as an Aid to Evaluate the Genetic Integrity of Somatic Embryogenesis-Derived Populations of Picea-Mariana (Mill) Bsp. Theoretical and Applied Genetics, 86(1):81-87.

Résumé

The usefulness of random amplified polymorphic DNA (RAPD) in assessing the genetic stability of somatic embryogenesis-derived populations of black spruce [Picea mariana (Mill.) B.S.P.] was evaluated. Three arbitrary 11-mer primers were successfully used to.amplify DNA from both in-vivo and in-vitro material. Twenty-five embryogenic cell lines, additional zygotic embryos and megagametophytes from three controlled crosses involving four selected genotypes of black spruce were used for the segregation analysis of RAPD variants. Ten markers were genetically characterized and used to evaluate the genetic stability of somatic embryos derived from three embryogenic cell lines (one cell line per cross, 30 somatic embryos per cell line). No variation was detected within clones. The utilization of RAPD markers both for the assessment of genetic stability of clonal materials and to certify genetic stability throughout the process of somatic embryogenesis is discussed.

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@ARTICLE { IsabelTremblayMichaudEtAl1993,
    AUTHOR = { Isabel, N. and Tremblay, L. and Michaud, M. and Tremblay, F.M. and Bousquet, J. },
    TITLE = { Rapds as an Aid to Evaluate the Genetic Integrity of Somatic Embryogenesis-Derived Populations of Picea-Mariana (Mill) Bsp },
    JOURNAL = { Theoretical and Applied Genetics },
    YEAR = { 1993 },
    VOLUME = { 86 },
    PAGES = { 81-87 },
    NUMBER = { 1 },
    NOTE = { Times Cited: 89 Article English Cited References Count: 46 Kv862 },
    ABSTRACT = { The usefulness of random amplified polymorphic DNA (RAPD) in assessing the genetic stability of somatic embryogenesis-derived populations of black spruce [Picea mariana (Mill.) B.S.P.] was evaluated. Three arbitrary 11-mer primers were successfully used to.amplify DNA from both in-vivo and in-vitro material. Twenty-five embryogenic cell lines, additional zygotic embryos and megagametophytes from three controlled crosses involving four selected genotypes of black spruce were used for the segregation analysis of RAPD variants. Ten markers were genetically characterized and used to evaluate the genetic stability of somatic embryos derived from three embryogenic cell lines (one cell line per cross, 30 somatic embryos per cell line). No variation was detected within clones. The utilization of RAPD markers both for the assessment of genetic stability of clonal materials and to certify genetic stability throughout the process of somatic embryogenesis is discussed. },
    KEYWORDS = { rapd picea-mariana genetic markers genetic stability somatic embryogenesis heritable somaclonal variation tissue-culture interior spruce norway spruce DNA amplification anther culture abscisic-acid white spruce plants embryos },
    OWNER = { brugerolles },
    TIMESTAMP = { 2007.12.05 },
}

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