GosselinZhouBousquetEtAl2002

Référence

Gosselin, I., Zhou, Y., Bousquet, J., Isabel, N. (2002) Megagametophyte-derived linkage maps of white spruce (Picea glauca) based on RAPD, SCAR and ESTP markers. Theoretical and Applied Genetics, 104(6-7):987-997.

Résumé

We have constructed linkage maps for two parents of white spruce [Picea glauca (Moench) Voss]. Haploid megagametophytes from 92 and 96 seeds of parents M2 and 80132, respectively, were analysed with RAPD, SCAR and ESTP markers. Fragments segregating in a 1: 1 Mendelian ratio were classified and mapped using MAPMAKER, GMENDEL and JOINMAP. For M2, the analysis with JOINMAP resulted in 165 loci (152 RAPDs, 3 SCARs and 10 ESTPs) mapping to 23 linkage groups and covering 2,059.4 cM(Kosambi function, K). For 80132, the analysis resulted in 144 loci (137 RAPDs, I SCAR and 7 ESTPs) mapping to 19 linkage groups and covering 2,007.7 cM(K). The maps covered 87 and 73% of the entire genome of parents M2 and 80132, respectively. Similar results were obtained with MAPMAKER and GMENDEL. A comparison was made between the two individual maps and 16 loci were shared between the two maps.

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@ARTICLE { GosselinZhouBousquetEtAl2002,
    AUTHOR = { Gosselin, I. and Zhou, Y. and Bousquet, J. and Isabel, N. },
    TITLE = { Megagametophyte-derived linkage maps of white spruce (Picea glauca) based on RAPD, SCAR and ESTP markers },
    JOURNAL = { Theoretical and Applied Genetics },
    YEAR = { 2002 },
    VOLUME = { 104 },
    PAGES = { 987-997 },
    NUMBER = { 6-7 },
    NOTE = { Times Cited: 11 Article English Cited References Count: 49 560lh },
    ABSTRACT = { We have constructed linkage maps for two parents of white spruce [Picea glauca (Moench) Voss]. Haploid megagametophytes from 92 and 96 seeds of parents M2 and 80132, respectively, were analysed with RAPD, SCAR and ESTP markers. Fragments segregating in a 1: 1 Mendelian ratio were classified and mapped using MAPMAKER, GMENDEL and JOINMAP. For M2, the analysis with JOINMAP resulted in 165 loci (152 RAPDs, 3 SCARs and 10 ESTPs) mapping to 23 linkage groups and covering 2,059.4 cM(Kosambi function, K). For 80132, the analysis resulted in 144 loci (137 RAPDs, I SCAR and 7 ESTPs) mapping to 19 linkage groups and covering 2,007.7 cM(K). The maps covered 87 and 73% of the entire genome of parents M2 and 80132, respectively. Similar results were obtained with MAPMAKER and GMENDEL. A comparison was made between the two individual maps and 16 loci were shared between the two maps. },
    KEYWORDS = { codominant markers conifers genetic linkage map gmendel joinmap mapmaker fragment length polymorphisms site sts markers pine pinus-pinaster arbitrary genes black spruce pseudo-testcross genome length norway spruce douglas-fir taeda l. },
    OWNER = { brugerolles },
    TIMESTAMP = { 2007.12.05 },
}

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