RiouxPelletierSt-Laurent2019

Référence

Rioux, È., Pelletier, F., St-Laurent, M.-H. (2019) Influence of lipids on stable isotope ratios in mammal hair: highlighting the importance of validation. Ecosphere, 10(5). (Scopus )

Résumé

Carbon (δ13C) and nitrogen (δ15N) stable isotope ratios are increasingly used in ecological studies to evaluate diet composition and trophic relationships. However, lipids may influence stable isotope ratios due to the depletion of 13C in adipose tissues relative to proteins and carbohydrates. δ13C values can be corrected by lipid extraction or normalization models. The aims of our study were to evaluate the effects of lipid extraction on stable isotope ratios in a terrestrial mammal, the caribou (Rangifer tarandus caribou), and to propose relevant lipid normalization models that are method- and tissue-specific for δ13C values. We also evaluated whether four δ13C lipid normalization and correction models proposed in the literature were applicable to our study species. Stable isotope ratios were obtained for hair, plasma, and red blood cell samples of 44 caribou in the Gaspésie National Park (Québec, Canada). The effects of lipid extraction on stable isotope ratios were tested using a paired t-test. A simple linear model was used to correct for the effects of lipid extraction and to assess its performance compared to that of published equations. Lipid content significantly influenced δ13C values in caribou hair. The four lipid normalization equations commonly used in the literature did not accurately predict δ13Clipid-free values of caribou hair. Based on our results, we recommend controlling systematically for lipids in terrestrial systems and analyzing δ13C (lipid-free) and δ15N (bulk) from two separate aliquots to reach a greater precision. We also recommend controlling for lipids in hair tissue. If not possible, we recommend using a lipid normalization model that is tissue-, method-, and species-specific or applying a model that has been previously validated for the tissue and species of interest. © 2019 The Authors.

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@ARTICLE { RiouxPelletierSt-Laurent2019,
    AUTHOR = { Rioux, È. and Pelletier, F. and St-Laurent, M.-H. },
    TITLE = { Influence of lipids on stable isotope ratios in mammal hair: highlighting the importance of validation },
    JOURNAL = { Ecosphere },
    YEAR = { 2019 },
    VOLUME = { 10 },
    NUMBER = { 5 },
    NOTE = { cited By 0 },
    ABSTRACT = { Carbon (δ13C) and nitrogen (δ15N) stable isotope ratios are increasingly used in ecological studies to evaluate diet composition and trophic relationships. However, lipids may influence stable isotope ratios due to the depletion of 13C in adipose tissues relative to proteins and carbohydrates. δ13C values can be corrected by lipid extraction or normalization models. The aims of our study were to evaluate the effects of lipid extraction on stable isotope ratios in a terrestrial mammal, the caribou (Rangifer tarandus caribou), and to propose relevant lipid normalization models that are method- and tissue-specific for δ13C values. We also evaluated whether four δ13C lipid normalization and correction models proposed in the literature were applicable to our study species. Stable isotope ratios were obtained for hair, plasma, and red blood cell samples of 44 caribou in the Gaspésie National Park (Québec, Canada). The effects of lipid extraction on stable isotope ratios were tested using a paired t-test. A simple linear model was used to correct for the effects of lipid extraction and to assess its performance compared to that of published equations. Lipid content significantly influenced δ13C values in caribou hair. The four lipid normalization equations commonly used in the literature did not accurately predict δ13Clipid-free values of caribou hair. Based on our results, we recommend controlling systematically for lipids in terrestrial systems and analyzing δ13C (lipid-free) and δ15N (bulk) from two separate aliquots to reach a greater precision. We also recommend controlling for lipids in hair tissue. If not possible, we recommend using a lipid normalization model that is tissue-, method-, and species-specific or applying a model that has been previously validated for the tissue and species of interest. © 2019 The Authors. },
    AFFILIATION = { Centre for Forest Research, Département de Biologie, Chimie et Géographie, Université du Québec à Rimouski, 300 Allée des Ursulines, Rimouski, QC G5L 3A1, Canada; Canada Research Chair in Evolutionary Demography and Conservation, Centre for Northern Studies, Département de biologie, Université de Sherbrooke, 2500 Boulevard de l'Université, Sherbrooke, QC J1K 2R1, Canada },
    ART_NUMBER = { e02723 },
    AUTHOR_KEYWORDS = { lipid correction; lipid extraction; mathematical normalization; Rangifer tarandus caribou; stable isotopes; terrestrial ecosystems; δ13C; δ15N },
    DOCUMENT_TYPE = { Article },
    DOI = { 10.1002/ecs2.2723 },
    SOURCE = { Scopus },
    URL = { https://www.scopus.com/inward/record.uri?eid=2-s2.0-85066477828&doi=10.1002%2fecs2.2723&partnerID=40&md5=9ff42a278d523cd191e4c6412604bfe4 },
}

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