JugeChampagneCoughlanEtAl2009

Référence

Juge, C., Champagne, A., Coughlan, A.P., Juge, N., Parrott, L., Piche, Y. (2009) Quantifying the growth of arbuscular mycorrhizal fungi: usefulness of the fractal dimension. Botany, 87(4):387-400.

Résumé

The present study is, to the best of our knowledge, the first to investigate the use of the fractal dimension (FD) to quantify the growth and development of undisturbed, fully functional arbuscular mycorrhizal (AM) hyphae developing in vitro. The majority of the work focused on the model AM fungus Glomus intraradices DAOM 181602. The time course study and final measurements of an intact mature extraradical mycelium allowed us to compare the development of the mycelium and the FD value. The final FD value of 1.62 for the mature mycelium is similar to that obtained for highly branched root systems and tree crowns. The FD method was used to characterize the morphology of germinative and pre-symbiotic hyphae in the presence of stimulatory (strigolactone GR-24, 0.1 mu mol(.)L(-1) and bisphenol A, 10 mu mol(.)L(-1)) and inhibitory (NaCl, 80 mmol(.)L(-1)) molecules, and the extraradical phase in the presence of an inhibitory molecule (NaCl, 80 mmol(.)L(-1)). Where possible, results were compared with those obtained using the traditional grid-line (GL) technique. The FD approach allowed treatment effects to be accurately quantified, both in germinative and extraradical phases. In the second case, this technique provided a single quantitative value of extraradical hyphal growth that included runner hyphae (RH) networks, and fine-branching (FB) ramifications. This is in contrast to the GL technique, which provides a value for the estimation of RH, but which is not suitable for accurately measuring FB hyphae. Given the ease with which the FD values can be calculated, and the fact that this method can provide a single value for the quantification of extraradical hyphal growth and development, we suggest that this method is useful for in vitro studies. Furthermore under certain situations of germinative or presymbiotic growth, it may be used in concert with the GL method to provide a greater degree of information about hyphal morphology. The usefulness and limits of the FD method at different stages of the AM fungal growth cycle are discussed.

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@ARTICLE { JugeChampagneCoughlanEtAl2009,
    AUTHOR = { Juge, C. and Champagne, A. and Coughlan, A.P. and Juge, N. and Parrott, L. and Piche, Y. },
    TITLE = { Quantifying the growth of arbuscular mycorrhizal fungi: usefulness of the fractal dimension },
    JOURNAL = { Botany },
    YEAR = { 2009 },
    VOLUME = { 87 },
    PAGES = { 387-400 },
    NUMBER = { 4 },
    MONTH = { apr },
    AF = { Juge, ChristineEOLEOLChampagne, AnnieEOLEOLCoughlan, Andrew P.EOLEOLJuge, NicolasEOLEOLParrott, LaelEOLEOLPiche, Yves },
    C1 = { [Juge, Christine; Champagne, Annie; Coughlan, Andrew P.; Piche, Yves] Univ Laval, Ctr Etud Foret, Quebec City, PQ G1V 0A6, Canada.EOLEOL[Juge, Nicolas] Univ Laval, Ctr Bioinformat & Biol Computat, Ste Foy, PQ G1K 7P4, Canada.EOLEOL[Parrott, Lael] Univ Montreal, Dept Geog, Lab Syst Complexes, Montreal, PQ H3C 3J7, Canada. },
    DE = { hyphal growth; fine branching (FB); runner hyphae (RH); grid-lineEOLEOLintersect (GL); Glomus intraradices; Glomus diaphanum },
    DI = { 10.1139/B09-006 },
    EM = { christine.juge@agr.gc.ca },
    FU = { Universite Laval ; Canadian Oil Sands Network for Research andEOLEOLDevelopment ; Fonds Quebecquois de la Recherche sur la Nature et lesEOLEOLTechnologies ; Natural Sciences and Engineering Research Council ofEOLEOLCanada },
    FX = { The authors thank Universite Laval and the Canadian Oil Sands NetworkEOLEOLfor Research and Development for the financial support provided to C.EOLEOLJ., and the Fonds Quebecquois de la Recherche sur la Nature et lesEOLEOLTechnologies and the Natural Sciences and Engineering Research CouncilEOLEOLof Canada for financial support to Y. P. and L. P. We thank MarcEOLEOLStArnaud for comments and advice on a previous draft of the manuscript. },
    GA = { 449ZH },
    J9 = { BOTANY },
    JI = { Botany },
    LA = { English },
    NR = { 66 },
    PA = { BUILDING M 55, OTTAWA, ON K1A 0R6, CANADA },
    PG = { 14 },
    PI = { OTTAWA },
    RP = { Juge, C, Ctr Rech & Dev Sols & Grandes Cultures, 2560 Blvd Hochelaga,EOLEOLSte Foy, PQ G1V 2J3, Canada. },
    SC = { Plant Sciences },
    SN = { 1916-2790 },
    TC = { 0 },
    UT = { ISI:000266369200005 },
    ABSTRACT = { The present study is, to the best of our knowledge, the first to investigate the use of the fractal dimension (FD) to quantify the growth and development of undisturbed, fully functional arbuscular mycorrhizal (AM) hyphae developing in vitro. The majority of the work focused on the model AM fungus Glomus intraradices DAOM 181602. The time course study and final measurements of an intact mature extraradical mycelium allowed us to compare the development of the mycelium and the FD value. The final FD value of 1.62 for the mature mycelium is similar to that obtained for highly branched root systems and tree crowns. The FD method was used to characterize the morphology of germinative and pre-symbiotic hyphae in the presence of stimulatory (strigolactone GR-24, 0.1 mu mol(.)L(-1) and bisphenol A, 10 mu mol(.)L(-1)) and inhibitory (NaCl, 80 mmol(.)L(-1)) molecules, and the extraradical phase in the presence of an inhibitory molecule (NaCl, 80 mmol(.)L(-1)). Where possible, results were compared with those obtained using the traditional grid-line (GL) technique. The FD approach allowed treatment effects to be accurately quantified, both in germinative and extraradical phases. In the second case, this technique provided a single quantitative value of extraradical hyphal growth that included runner hyphae (RH) networks, and fine-branching (FB) ramifications. This is in contrast to the GL technique, which provides a value for the estimation of RH, but which is not suitable for accurately measuring FB hyphae. Given the ease with which the FD values can be calculated, and the fact that this method can provide a single value for the quantification of extraradical hyphal growth and development, we suggest that this method is useful for in vitro studies. Furthermore under certain situations of germinative or presymbiotic growth, it may be used in concert with the GL method to provide a greater degree of information about hyphal morphology. The usefulness and limits of the FD method at different stages of the AM fungal growth cycle are discussed. },
    KEYWORDS = { GLOMUS-INTRARADICES; MYCELIAL SYSTEMS; GENERAL-MODEL; VASCULAR PLANTS; IMAGE-ANALYSIS; SOIL-SALINITY; HYPHAL GROWTH; IN-VIVO; ROOT; SYMBIOSIS },
    OWNER = { sobru1 },
    PUBLISHER = { Natl Research Council Canada-N R C Research Press },
    TIMESTAMP = { 2009.06.22 },
}

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