LeveeMajorLevasseurEtAl2009

Référence

Levee, V., Major, I., Levasseur, C., Tremblay, L., MacKay, J., Seguin, A. (2009) Expression profiling and functional analysis of Populus WRKY23 reveals a regulatory role in defense. New Phytologist, 184(1):48-70.

Résumé

WRKY transcription factors are key regulators that activate and fine-tune stress responses, including defense responses against pathogens. We isolated a poplar ( Populus tremula x Populus alba) cDNA sequence, PtWRKY23, that encodes the ortholog of Arabidopsis WRKY23 and present the functional analysis of WRKY23, with emphasis on its potential role in resistance to rust infection. To investigate the function of PtWRKY23, we examined PtWRKY23 expression after stress treatments by qRT-PCR and generated PtWRKY23-misexpressing plants. Transgenic plants were assessed for resistance to Melampsora rust and were analyzed using the poplar Affymetrix GeneChip (R) and histological techniques to study the consequences of PtWRKY23 misexpression. PtWRKY23 is rapidly induced by Melampsora infection and elicitor treatments and poplars overexpressing and underexpressing PtWRKY23 were both more susceptible to Melampsora infection than wild type. Transcriptome analysis of PtWRKY23 overexpressors revealed a significant overlap with the Melampsora-infection response. Transcriptome analysis also suggests that PtWRKY23 affects redox homeostasis and cell wall-related metabolism, which was confirmed by analyses that showed that PtWRKY23-misexpressing plants have altered peroxidase activity, apparent H2O2 accumulation and lignin deposition. Our results show that PtWRKY23 affects resistance to Melampsora infection and that this may be caused by deregulation of genes that disrupt redox homeostasis and cell wall metabolism.

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@ARTICLE { LeveeMajorLevasseurEtAl2009,
    AUTHOR = { Levee, V. and Major, I. and Levasseur, C. and Tremblay, L. and MacKay, J. and Seguin, A. },
    TITLE = { Expression profiling and functional analysis of Populus WRKY23 reveals a regulatory role in defense },
    JOURNAL = { New Phytologist },
    YEAR = { 2009 },
    VOLUME = { 184 },
    PAGES = { 48-70 },
    NUMBER = { 1 },
    AF = { Levee, ValerieEOLEOLMajor, IanEOLEOLLevasseur, CarolineEOLEOLTremblay, LaurenceEOLEOLMacKay, JohnEOLEOLSeguin, Armand },
    C1 = { [Levee, Valerie; Major, Ian; Levasseur, Caroline; Tremblay, Laurence; Seguin, Armand] Nat Resources Canada, Canadian Forest Serv, Laurentian Forestry Ctr, Quebec City, PQ G1V 4C7, Canada.EOLEOL[MacKay, John] Univ Laval, Fac Foresterie & Geomat, Quebec City, PQ G1K 7P4, Canada. },
    DE = { biotroph; forest tree; fungal pathogen; genomics; transcriptomic; WRKY },
    DI = { 10.1111/j.1469-8137.2009.02955.x },
    EM = { armand.seguin@nrcan.gc.ca },
    FU = { Natural Sciences and Engineering Research Council of Canada ; GenomeEOLEOLQuebec and Genome Canada },
    FX = { The authors thank Pamela Cheers for editing work and the knowledgeableEOLEOLreviewers for their constructive criticism. IM is the recipient of aEOLEOLpostdoctoral Visiting Fellowship in Canadian Government LaboratoriesEOLEOLfrom the Natural Sciences and Engineering Research Council of Canada.EOLEOLThis work was supported by grants from Genome Quebec and Genome CanadaEOLEOLto AS and JM for the Arborea project and the Canadian Genomics R&DEOLEOLInitiative to AS. },
    GA = { 490XO },
    J9 = { NEW PHYTOL },
    JI = { New Phytol. },
    LA = { English },
    NR = { 115 },
    PA = { COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA },
    PG = { 23 },
    PI = { MALDEN },
    RP = { Seguin, A, Nat Resources Canada, Canadian Forest Serv, LaurentianEOLEOLForestry Ctr, 1055 PEPS,POB 10380, Quebec City, PQ G1V 4C7, Canada. },
    SC = { Plant Sciences },
    SN = { 0028-646X },
    TC = { 0 },
    UT = { ISI:000269541600009 },
    ABSTRACT = { WRKY transcription factors are key regulators that activate and fine-tune stress responses, including defense responses against pathogens. We isolated a poplar ( Populus tremula x Populus alba) cDNA sequence, PtWRKY23, that encodes the ortholog of Arabidopsis WRKY23 and present the functional analysis of WRKY23, with emphasis on its potential role in resistance to rust infection. To investigate the function of PtWRKY23, we examined PtWRKY23 expression after stress treatments by qRT-PCR and generated PtWRKY23-misexpressing plants. Transgenic plants were assessed for resistance to Melampsora rust and were analyzed using the poplar Affymetrix GeneChip (R) and histological techniques to study the consequences of PtWRKY23 misexpression. PtWRKY23 is rapidly induced by Melampsora infection and elicitor treatments and poplars overexpressing and underexpressing PtWRKY23 were both more susceptible to Melampsora infection than wild type. Transcriptome analysis of PtWRKY23 overexpressors revealed a significant overlap with the Melampsora-infection response. Transcriptome analysis also suggests that PtWRKY23 affects redox homeostasis and cell wall-related metabolism, which was confirmed by analyses that showed that PtWRKY23-misexpressing plants have altered peroxidase activity, apparent H2O2 accumulation and lignin deposition. Our results show that PtWRKY23 affects resistance to Melampsora infection and that this may be caused by deregulation of genes that disrupt redox homeostasis and cell wall metabolism. },
    KEYWORDS = { CYTOSOLIC ASCORBATE PEROXIDASE-1; SYSTEMIC ACQUIRED-RESISTANCE; CARBOHYDRATE-ACTIVE ENZYMES; MELAMPSORA-LARICI-POPULINA; CLASS-III PEROXIDASES; DNA-BINDING PROTEINS; OXYGEN GENE NETWORK; TRANSCRIPTION FACTOR; PLANT DEFENSE; MAP KINASE },
    OWNER = { sobru1 },
    PUBLISHER = { Wiley-Blackwell Publishing, Inc },
    TIMESTAMP = { 2009.09.21 },
}

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