HarrisonBushPlettEtAl2007

Référence

Harrison, E.J., Bush, M., Plett, J.M., McPhee, D.P., Vitez, R., O'Malley, B., Sharma, V., Bosnich, W., Seguin, A., MacKay, J., Regan, S. (2007) Diverse developmental mutants revealed in an activation-tagged population of poplar. Canadian Journal of Botany, 85(11):1071-1081.

Résumé

We have produced the largest population of activation-tagged poplar trees to date, approximately 1800 independent lines, and report on phenotypes of interest that have been identified in tissue culture and greenhouse conditions. Activation tagging is an insertional mutagenesis technique that results in the dominant upregulation of an endogenous gene. A large-scale Agrobacterium-mediated transformation protocol was used to transform the pSKI074 activation-tagging vector into Populus tremula x Populus alba hybrid poplar. We have screened the first 1000 lines for developmental abnormalities and have a visible mutant frequency of 2.4%, with alterations in leaf and stem structure as well as overall stature. Most of the phenotypes represent new phenotypes that have not previously been identified in poplar and, in some cases, not in any other plant either. Molecular analysis of the T-DNA inserts of a subpopulation of mutant lines reveal both single and double T-DNA inserts with double inserts snore common in lines with visible phenotypes. The broad range of developmental mutants identified in this pilot screen of the population reveals that it will be a valuable resource for gene discovery in poplar. The full value of this population will only be realized as we screen these lines for a wide range of phenotypes.

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@ARTICLE { HarrisonBushPlettEtAl2007,
    AUTHOR = { Harrison, E.J. and Bush, M. and Plett, J.M. and McPhee, D.P. and Vitez, R. and O'Malley, B. and Sharma, V. and Bosnich, W. and Seguin, A. and MacKay, J. and Regan, S. },
    TITLE = { Diverse developmental mutants revealed in an activation-tagged population of poplar },
    JOURNAL = { Canadian Journal of Botany },
    YEAR = { 2007 },
    VOLUME = { 85 },
    PAGES = { 1071-1081 },
    NUMBER = { 11 },
    MONTH = { nov },
    AF = { Harrison, Edward J.EOLEOLBush, MichaelEOLEOLPlett, Jonathan M.EOLEOLMcPhee, Daniel P.EOLEOLVitez, RobinEOLEOLO'Malley, BrendanEOLEOLSharma, VijayaEOLEOLBosnich, WhynnEOLEOLSeguin, ArmandEOLEOLMacKay, JohnEOLEOLRegan, Sharon },
    DE = { developmental mutant; transgenic; functional genomics; Populus tremulaEOLEOLx Populus alba },
    PG = { 11 },
    SN = { 0008-4026 },
    TC = { 0 },
    UT = { ISI:000252318000008 },
    ABSTRACT = { We have produced the largest population of activation-tagged poplar trees to date, approximately 1800 independent lines, and report on phenotypes of interest that have been identified in tissue culture and greenhouse conditions. Activation tagging is an insertional mutagenesis technique that results in the dominant upregulation of an endogenous gene. A large-scale Agrobacterium-mediated transformation protocol was used to transform the pSKI074 activation-tagging vector into Populus tremula x Populus alba hybrid poplar. We have screened the first 1000 lines for developmental abnormalities and have a visible mutant frequency of 2.4%, with alterations in leaf and stem structure as well as overall stature. Most of the phenotypes represent new phenotypes that have not previously been identified in poplar and, in some cases, not in any other plant either. Molecular analysis of the T-DNA inserts of a subpopulation of mutant lines reveal both single and double T-DNA inserts with double inserts snore common in lines with visible phenotypes. The broad range of developmental mutants identified in this pilot screen of the population reveals that it will be a valuable resource for gene discovery in poplar. The full value of this population will only be realized as we screen these lines for a wide range of phenotypes. },
    KEYWORDS = { EXPRESSED SEQUENCE TAGS; AGROBACTERIUM-MEDIATED TRANSFORMATION; CINNAMYL ALCOHOL-DEHYDROGENASE; O-METHYLTRANSFERASE ACTIVITY; TRANSGENIC POPLAR; INSERTIONAL MUTAGENESIS; LIGNIN BIOSYNTHESIS; DOWN-REGULATION; ARABIDOPSIS; GENE },
    OWNER = { brugerolles },
    TIMESTAMP = { 2008.02.08 },
}

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