NaydenovTremblayBergeronEtAl2005

Référence

Naydenov, K.D., Tremblay, M.F., Bergeron, Y., Alexandrov, A. and Fenton, N. (2005) Dissimilar patterns of Pinus heldreichii Christ. populations in Bulgaria revealed by chloroplast microsatellites and terpenes analysis. Biochemical Systematics and Ecology, 33(2):133-148.

Résumé

In the present study we investigated the genetic structure and genetic diversity of Pinus heldreichii populations in Bulgaria using chloroplast microsatellite markers and terpene analysis. We were interested in addressing the following questions: (1) can population structuring in Bosnian pine be detected via chloroplast microsatellite markers; (2) are there differences in population differentiation as determined by terpenes and microsatellites; and (3) how are the patterns of size variant frequencies and geographical distances related. Four provenances were chosen throughout the species' range in Bulgaria. Following DNA extraction, chloroplast microsatellite (cpSSR) loci were surveyed using 6 primer pairs. Between 2 and 5 size variants were identified at each locus. A total of 16 size variants at the 6 loci were identified, 4 occurring at low frequencies. They were combined in 21 different haplotypes including 11 that were unique. AMOVA analysis revealed that 18.25% of the variation was found among populations, while 81.75% was expressed within populations. The cpSSR analysis divided Bosnian pine populations into two groups, the first represented by populations B, C and D located in the south and north-western part of the Pirin and Slavianka mountains, while the second group, represented by population A, is located in the north-eastern Pirin mountain. Terpene analysis revealed that on average, 59% of the monoterpene pool in P. heldreichii is accounted for by limonene (range 36-48%) followed by ?-pinene (range 16-17%). The presence of two distinct groups (Pop-A, Pop-D and Pop-B, Pop-C) is more consistent with physical distances between populations. No significant correlation between genetic distance determined by chloroplast microsatellites analysis and chemotype distance determined by terpenes was observed. Our results suggest that the structural pattern of genetic diversity of cpDNA in Bosnian pine populations is the consequence of historical and biogeographical processes. © 2004 Elsevier Ltd. All rights reserved.

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@ARTICLE { NaydenovTremblayBergeronEtAl2005,
    AUTHOR = { Naydenov, K.D. and Tremblay, M.F. and Bergeron, Y. and Alexandrov, A. and Fenton, N. },
    TITLE = { Dissimilar patterns of Pinus heldreichii Christ. populations in Bulgaria revealed by chloroplast microsatellites and terpenes analysis },
    JOURNAL = { Biochemical Systematics and Ecology },
    YEAR = { 2005 },
    VOLUME = { 33 },
    PAGES = { 133-148 },
    NUMBER = { 2 },
    NOTE = { 03051978 (ISSN) Cited By (since 1996): 1 Export Date: 25 April 2007 Source: Scopus CODEN: BSECB doi: 10.1016/j.bse.2004.06.017 Language of Original Document: English Correspondence Address: Chaire CRSNG-UQAT-UQAM A.; Univ. Quebec Abitibi-Temiscamingue; 445 Boul. l'Univ., Rouyn-Noranda, Q.email: krassimirdn@hotmail.com References: Adams, R., Systematic of multi-seeded eastern hemisphere Juniperus based of leaf essential oils and RAPD DNA fingerprinting (1999) Biochem. Syst. Ecol., 27, pp. 709-725; Adams, R., Systematics of smooth leaf margin Juniperus of the western hemisphere based of leaf essential oils and RAPD DNA fingerprinting (2000) Biochem. Syst. Ecol., 28, pp. 149-162; Adams, R., Systematics of Juniperus section Juniperus based of leaf essential oils and RAPD DNA fingerprinting (2000) Biochem. Syst. Ecol., 28, pp. 515-528; Adams, R., Systematics of the one seeded Juniperus of the eastern hemisphere based of leaf essential oils and RAPD DNA fingerprinting (2000) Biochem. Syst. Ecol., 28, pp. 529-543; Adams, R., The serrate leaf margined Juniperus (Section Sabina) of the western hemisphere: Systematic and evolution based on leaf essential oil and Random Amplified Polymorphic DNAs (RAPDs) (2000) Biochem. Syst. Ecol., 28, pp. 975-989; Adams, R., Schwarzbach, A., Pandey, R., The concordance of terpenoid, ISSR and RAPD markers, and UTS sequence data set among genotypes: An example from Juniperus (2003) Biochem. Syst. Ecol., 31, pp. 375-387; Alexandrov, A., Rafailov, G., Nedelin, G., Canov, K., Bogdanov, B., Spasov, C., (1988) Coniferous Forest in Bulgaria, , Zemizdat Sofia; Alexandrov, A., Genetic Conservation of Conifers in Bulgaria (2000) J. Balkan Ecol., 3, pp. 5-10; Avolio, S., Il pino loricato (Pinus leucodermis Ant.) (1984) Ann. Ist. Sper. Selv., 17, pp. 79-153; Balevska, L., Alexandrov, A., Sur le polymorphisme des proteins solubles, l'esterase et la peroxydase dans les graines de Pinus leucodermis Ant. des montagne Pirine et Slavjanka (1972) Symposium de Pinus Heldreichii, pp. 22-28. , Pec., Kosovo; Belkhir, K., Genetix ver 4.01: A software for population genetics data analysis (2002) Loboratoire Genome et Populations, , http://www.univ-montp2.fr/~genetix/genetix.htm), Universite de Montpellier II France; Bocherini, G., Michelozzi, M., Gas-chromatography of terpene composition of Picea abies K (1993) XV International Symposium on Capillary Chromatography - May 1993, Riva del Garda, Italy, pp. 1329-1334. , P. Sandra G. Devos Dr. Alfred Huethig Publishing GmbH Heidelberg-Germany, II; Bosherini, G., Morgante, M., Rossi, P., Vendramin, G., Allozyme and chloroplast DNA variation in Italian and Greek populations of Pinus leucodermis (1994) Heredity, 73, pp. 284-290; Bonnin, I., Prosperi, J.-M., Olivieri, I., Genetic markers and quantitative genetic variation in Medicago truncatula (Leguminisa): A comparative analysis of population structure (1996) Genetics, 143, pp. 1795-1805; Butlin, R., Tregenza, T., Levels of genetic polymorphism: Marker loci versus quantitative traits (1998) Philos. Trans. R. Soc. Lond. B, 353, pp. 187-198; Chang, J., Hanover, J., Geographic variation in the monoterpene composition of Black Spruce (1991) Can. J. For. Res., 21, pp. 1796-1800; Critchfield, W.B., Little, E.L., (1966) Geographic Distribution of the Pines of the World, , U.S. Department of Agriculture Washington; Dobrinov, I., Doykov, G., Gagov, V., (1982) Forest Genetic Pool in Bulgaria, , Zemizdat Sofia; Dobrinov, I., (1983) Genetic and Selection of Forest Tree, , Zemizdat Sofia; Epperson, B., Fine-scale spatial structure: Correlations for individual genotypes differ from those for local gene frequencies (1995) Evolution, 49, pp. 1022-1026; Epperson, B., Li, T., Measurement of genetic structure within populations using Moran's spatial autocorrelation statistics (1996) Proc. Natl Acad. Sci. U.S.A., 93, pp. 10528-10532; Excoffier, L., Smouse, P., Quattro, J., Analysis of molecular variance inferred from metric distances among DNA haplotypes: Application to human mitochondrial DNA restriction data (1992) Genetics, 131, pp. 479-491; Excoffier, L., Schneider, S., Roessli, D., (2002) Arlequin Ver 2.001: A Software for Population Genetics Data Analysis, , http://anthrol.unige.ch/arlequin, Department of Anthropology and Ecology, University of Geneva Switzerland; Fady, B., Arbez, M., Marpeau, A., Geographic variability of terpene composition in Abies cephalonica London and Abies species around the Aegean: Hypotheses for their possible phylogeny from the Miocene (1992) Trees, 6, pp. 162-171; Forrest, G., Genotypic variation among native Scots pine population in Scotland based on monoterpene analysis (1990) Forestry, 53, pp. 101-128; Huttunen, A., Huttunen, R., Vasari, R., Panovska, H., Bozilova, E., Late-glacial and Holocene history of flora and vegetation in the western Rhodopes mountains, Bulgaria (1992) Acta Bot. Fenn., 144, pp. 63-80; Jaramillo-Correa, J., Beaulieu, J., Bousquet, J., Conrtasting evolutionary forces driving population structure at expressed sequence tag polymorphisms, allozymes and quantitative traits in white spruce (2001) Mol. Ecol., 10, pp. 2729-2740; Kalendar, R., (2002) Oligo 9.9. (Windows NT), [Computer Program Manual], , http://www.biocenter.helsinki.fi, Institute of Biotechnology, University of Helsinki Finland; Karhu, A., Hurme, P., Karjalainen, M., Karvonen, P., Karkkaunen, K., Neale, D., Savolainen, O., Do molecular markers reflect patterns of differentiation in adaptive traits of conifers? (1996) Theor. Appl. Genet., 93, pp. 215-221; Kuittinen, H., Mattila, A., Savolainene, O., Genetic variation at marker loci and in quantitative traits in natural populations of Arabidopsis thaliana (1997) Heredity, 79, pp. 144-152; Latta, R., Linhart, Y., Mitton, J., Cytonuclear disequilibrium and genetic drift in a Natural Population of Ponderosa pine (2001) Genetics, 158, pp. 843-850; Latta, R., Linhart, Y., Snyder, M., Lundquist, L., Patterns of variation and correlation in the monoterpene composition of xylem oleoresin within populations of ponderosa pine (2003) Biochem. Syst. Ecol., 31, pp. 451-465; Levin, R., McDate, L., Raguso, R., The systematic Utility of floral and vegetative fragrance in two genera of Nyctaginaceae (2003) Syst. Biol., 52, pp. 334-351; Lynch, M., Pfrender, M., Spitze, K., The quantitative and molecular genetic architecture of a subdivided species (1999) Evolution, 53, pp. 100-110; Mantel, N., The detection of disease clustering and a generalized regression approach (1967) Cancer Res., 27, pp. 209-220; Marshall, H., Newton, C., Ritland, K., Chloroplast phylogeography and evolution of highly polymorphic microsatellites in lodgepole pine (Pinus contorta) (2002) Theor. Appl. Genet., 104, pp. 367-372; McCauley, D., Contrasting the distribution of chloroplast DNA and allozyme polymorphism among local populations of Silene alba: Implications for studies of gene flow in plants (1994) Proc. Natl Acad. Sci. U.S.A., 91, pp. 8127-8131; Michalakis, Y., Excoffier, L., A generic estimate of population subdivision using distances between size variants with special reference to microsatellite loci (1996) Genetics, 142, pp. 1061-1064; Mitton, J., Kreiser, B., Latta, R., Glacial refugia of limber pine (Pinus flexilis James) inferred from the population structure of mitochondrial DNA (2000) Mol. Ecol., 9, pp. 91-97; Morgante, M., Vendramin, G., Olivieri, A., Mating system analysis in Pinus leucodermis Ant.: Detection of self-fertilization in natural populations (1991) Heredity, 67, pp. 197-203; Morgante, M., Vendramin, G., Giannini, R., Inheritance and linkage relationships of isozyme variants of Pinus leucodermis Ant (1993) Silvae Genet., 42, pp. 231-236; Naydenov, K., Velkov, D., Alexandrov, A., Genov, K., Asparuchova, E., Iliev, I., (1993) Research of Chemiphenotipic Variation of Representatives of Gender PINUS in Regards to Their Saving, , Rapport N CC-318/93 NFNI-MONT, Bulgaria; Naydenov, K., Markov, G., Variability and similarity of the terpene compositions of the essential oils in natural stands in Bulgaria of palebark heldreichii pine (Pinus heldreichii Christ.) (1995) Biochemical Markers in the Population Genetics of Forests Trees, pp. 165-170. , Ph. Baradat T. Adams G. Muller-Starck SPB Academic Publishing Amsterdam, The Netherlands; Naydenov, K.D., Co-relationship between terpenes in the essential oil of Palebark Heldreichii pine (Pinus heldreichii Christ.), determined through CGC in different provenances in Bulgaria (1996) XVIII International Symposium on Capillary Chromatography - May 1996, Riva del Garda, Italy, pp. 1247-1251. , P. Sandra G. Devos Dr. Alfred Huethig Publishing GmbH Heidelberg-Germany, II; Naydenov, K.D., Co-relationship between terpenes in the essential oil of Scotch pine Pinus sylvestris L., determined through CGC in different provenances in Bulgaria (1996) XVIII International Symposium on Capillary Chromatography - May 1996, Riva del Garda, Italy, pp. 1242-1246. , P. Sandra G. Devos Dr. Alfred Huethig Publishing GmbH Heidelberg-Germany, II; Naydenov, K., (1998) Phenotypic Diversity in Some Populations of Scots Pine (Pinus sylvestris L.) in the Rila, Pirin and the Rhodopes Mountains, Determined by Gas-chromatography of Terpenes, , Ph.D. thesis of forest genetic, selection and tree breeding. Bulgarian Academy of Sciences, Sofia, Bulgaria; Nei, M., Estimation of average heterozygosity and genetic distance from a small number of individuals (1978) Genetics, 89, pp. 583-590; Orive, M., Asmussen, M., The effects of pollen and seed migration on nuclear-dicytoplasmic systems. II. A new method for estimating plant gene flow from joint nuclear-cytoplasmic data (2000) Genetics, 155, pp. 833-854; Ozenda, P., Sur les e?tages de ve?ge?tation dans les montagnes du bassin me?diterrane?em. Documents Cartographique Ecologiques (1975) Grenoble, 16, pp. 1-32; Ozenda, P., (1985) La Ve?ge?tation de la Chai?ne Alpine dans l'Espace Montagnard Europe?en, , Masson Paris; Petit, R., Bahrman, N., Baradat, Ph., Comparison of genetic differentiation in maritime pine (Pinus pinaster Ait.) estimated using isozyme, total protein and terpenic loci (1995) Heredity, 75, pp. 382-389; Powell, W., Morgante, M., McDevitt, R., Vendramin, G., Rafalski, J., Polymorphic simple sequence repeat regions in chloroplast genomes application to the populations genetics of pines (1995) Proc. Natl Acad. Sci. U.S.A., 92, pp. 7759-7763; Ribeiro, M., Mariette, S., Vendramin, G., Szmidt, E., Plomion, C., Kremer, A., Comparison of genetic diversity estimates within and among populations of maritime pine using chloroplast simple-sequence repeat and amplified fragment length polymorphism data (2002) Mol. Ecol., 11, pp. 869-877; Rogers, A., Population differences in quantitative characters as opposed to gene frequencies (1986) Am. Nat., 127, pp. 729-730; Sandra, P., Bicchi, C., (1987) Capillary Gas Chromatography in Essential Oil Analysis, , Dr. Alfred Huethig Publishing GmbH Heidelberg-Germany; Schonwitz, R., Kloos, M., Merk, L., Zeigler, H., Patterns of monoterpenes stored in the needles of Picea abies L. Karst. from several locations in mountainous regions of Southern Germany (1990) Trees, 4, pp. 27-33; Slatkin, M., Gene flow and geographic structure of natural populations (1987) Science (Washington, D.C.), 236, pp. 787-792; Slatkin, M., Barton, N., A comparison of three indirect methods for estimating average levels of gene flow (1989) Evolution, 43, pp. 1349-1368; Slatkin, M., A measure of population subdivision based on microsatellite allele frequencies (1995) Genetics, 139, pp. 457-462; Spitze, K., Population structure in Daphnia obtusa: Quantitative genetics and allozymic variation (1993) Genetics, 135, pp. 367-374; (1999) STATISTICA for Windows (Computer Program Manual). 2300 East 14th Street, Tulsa, , http://www.statsoft.com; Stefanov, B., (1941) Geographical Distribution of Coniferous Species and Their Form in Nature, 1920. , Godichnik na Sofiiskia Darjaven Universitet Sofia, Bulgaria; Stefanov, B., (1943) The Phito-geographical Elements of Bulgaria, 39. , Thesis of Bulgarian Academy of Sciences, Faculty of Nature and Mathematics, N19, Sofia, Bulgaria; Strauss, S., Hong, Y.-P., Hipkins, V., High levels of population differentiation for mitochondrial DNA haplotypes in Pinus radiata, muricata and attenuata (1993) Theor. Appl. Genet., 86, pp. 605-611; Vendramin, G., Lelli, L., Rossi, P., Morgante, M., A set of primer for the amplification of 20 chloroplast microsatellites in Pinaceae (1996) Mol. Ecol., 5, pp. 585-598; Vendramin, G., Anzidei, M., Madaghiele, A., Bucci, G., Distribution of genetic diversity in Pinus pinaster Ait. as revealed by chloroplast microsatellites (1998) Theor. Appl. Genet., 97, pp. 456-463; Vendramin, G., Degen, B., Petit, R., Anzidel, M., Madaghiele, A., Ziegenhagen, B., High level of variation of Abies alba chloroplast microsatellite loci in Europe (1999) Mol. Ecol., 8, pp. 1117-1128; Vendramin, G., Anzidei, M., Madaghiele, A., Sperisen, C., Bucci, G., Chloroplast microsatellite analysis reveals the presence of population subdivision in Norway spruce (Picea abies K.) (2000) Genome, 43, pp. 68-78; Walter, R., Epperson, B., Geographic pattern of genetic variation in Pinus resinosa area of greatest diversity is not the origin of postglacial populations (2001) Mol. Ecol., 10, pp. 103-111; Weir, B., Cockerham, C., Estimating F-statistics for the analysis of population structure (1984) Evolution, 38, pp. 1123-1128; Yang, R.-C., Yeh, F., Tanchuk, A., A comparison of isozyme and quantitative genetic variation in Pinus contorta ssp. latifolia by Fst (1996) Genetics, 142, pp. 1045-1052; Zheng, Y., Ennos, R., Genetic variability and structure of natural and domesticated populations of Caribbean pine (Pinus caribaea Morelet) (1999) Theor. Appl. Genet., 98, pp. 765-771. },
    ABSTRACT = { In the present study we investigated the genetic structure and genetic diversity of Pinus heldreichii populations in Bulgaria using chloroplast microsatellite markers and terpene analysis. We were interested in addressing the following questions: (1) can population structuring in Bosnian pine be detected via chloroplast microsatellite markers; (2) are there differences in population differentiation as determined by terpenes and microsatellites; and (3) how are the patterns of size variant frequencies and geographical distances related. Four provenances were chosen throughout the species' range in Bulgaria. Following DNA extraction, chloroplast microsatellite (cpSSR) loci were surveyed using 6 primer pairs. Between 2 and 5 size variants were identified at each locus. A total of 16 size variants at the 6 loci were identified, 4 occurring at low frequencies. They were combined in 21 different haplotypes including 11 that were unique. AMOVA analysis revealed that 18.25% of the variation was found among populations, while 81.75% was expressed within populations. The cpSSR analysis divided Bosnian pine populations into two groups, the first represented by populations B, C and D located in the south and north-western part of the Pirin and Slavianka mountains, while the second group, represented by population A, is located in the north-eastern Pirin mountain. Terpene analysis revealed that on average, 59% of the monoterpene pool in P. heldreichii is accounted for by limonene (range 36-48%) followed by ?-pinene (range 16-17%). The presence of two distinct groups (Pop-A, Pop-D and Pop-B, Pop-C) is more consistent with physical distances between populations. No significant correlation between genetic distance determined by chloroplast microsatellites analysis and chemotype distance determined by terpenes was observed. Our results suggest that the structural pattern of genetic diversity of cpDNA in Bosnian pine populations is the consequence of historical and biogeographical processes. © 2004 Elsevier Ltd. All rights reserved. },
    KEYWORDS = { Biogeography Bosnian pine (syn. Heldreichii pine) Chloroplast Differentiation Microsatellites Pinus heldreichii Christ Population Terpenes biogeography differentiation genetic variation population genetics Bulgaria Eastern Hemisphere Eurasia Europe Pirin Mountains Slavianka Mountains Southern Europe World Pinus heldreichii },
    OWNER = { brugerolles },
    TIMESTAMP = { 2007.12.05 },
}

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