BrassardBrissetteLordEtAl1996

Référence

Brassard, N., Brissette, L., Lord, D. and Laliberte, S. (1996) Elongation, rooting and acclimatization of micropropagated shoots from mature material of hybrid larch. Plant Cell, Tissue and Organ Culture, 44(1):37-44.

Résumé

Factors were defined for elongation, rooting and acclimatization of micropropagated shoots of Larix x eurolepis Henry initiated from short shoot buds of plagiotropic stecklings serially propagated for 9 years from an 8-year-old tree. Initiation and multiplication were on Schenk and Hildebrandt (SH) medium supplemented with 5 ?M 6 - benzyladenine (BA) and 1 ?M indole-butyric acid (IBA). Stem elongation was obtained in 36% of the shoots on SH medium containing 0.5 ?M BA and 63% of the remaining non-elongated shoots initiated stem elongation after transfer on SH medium devoid of growth regulators. Rooting involved 2 steps: root induction on Campbell and Durzan mineral salts and Murashige and Skoog organic elements, both half-strength (CD-MS/2), supplemented with 1 ?M of both naphthaleneacetic acid (NAA) and IBA, and root elongation following transfer to CD-MS/2 medium devoid of growth regulators. Repeating this 2-step sequence yielded up to 67% rooted shoots. Acclimatization of plantlets ranged from 83% to 100%. Over 300 plants were transferred to the greenhouse; some showed plagiotropic growth. © 1996 Kluwer Academic Publishers.

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@ARTICLE { BrassardBrissetteLordEtAl1996,
    AUTHOR = { Brassard, N. and Brissette, L. and Lord, D. and Laliberte, S. },
    TITLE = { Elongation, rooting and acclimatization of micropropagated shoots from mature material of hybrid larch },
    JOURNAL = { Plant Cell, Tissue and Organ Culture },
    YEAR = { 1996 },
    VOLUME = { 44 },
    PAGES = { 37-44 },
    NUMBER = { 1 },
    ABSTRACT = { Factors were defined for elongation, rooting and acclimatization of micropropagated shoots of Larix x eurolepis Henry initiated from short shoot buds of plagiotropic stecklings serially propagated for 9 years from an 8-year-old tree. Initiation and multiplication were on Schenk and Hildebrandt (SH) medium supplemented with 5 ?M 6 - benzyladenine (BA) and 1 ?M indole-butyric acid (IBA). Stem elongation was obtained in 36% of the shoots on SH medium containing 0.5 ?M BA and 63% of the remaining non-elongated shoots initiated stem elongation after transfer on SH medium devoid of growth regulators. Rooting involved 2 steps: root induction on Campbell and Durzan mineral salts and Murashige and Skoog organic elements, both half-strength (CD-MS/2), supplemented with 1 ?M of both naphthaleneacetic acid (NAA) and IBA, and root elongation following transfer to CD-MS/2 medium devoid of growth regulators. Repeating this 2-step sequence yielded up to 67% rooted shoots. Acclimatization of plantlets ranged from 83% to 100%. Over 300 plants were transferred to the greenhouse; some showed plagiotropic growth. © 1996 Kluwer Academic Publishers. },
    KEYWORDS = { Acclimatization Conifer Larix x eurolepis Mature material Plagiotropism Rooting Stem elongation },
    OWNER = { racinep },
    TIMESTAMP = { 2007.09.07 },
}

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