GhaiSimonsChapmanEtAl2014

Référence

Ghai, R.R., Simons, N.D., Chapman, C.A., Omeja, P.A., Davies, T.J., Ting, N. and Goldberg, T.L. (2014) Hidden Population Structure and Cross-species Transmission of Whipworms (Trichuris sp.) in Humans and Non-human Primates in Uganda. PLoS Neglected Tropical Diseases, 8(10). (Scopus )

Résumé

Background:Whipworms (Trichuris sp.) are a globally distributed genus of parasitic helminths that infect a diversity of mammalian hosts. Molecular methods have successfully resolved porcine whipworm, Trichuris suis, from primate whipworm, T. trichiura. However, it remains unclear whether T. trichiura is a multi-host parasite capable of infecting a wide taxonomic breadth of primate hosts or a complex of host specific parasites that infect one or two closely related hosts. Methods and Findings:We examined the phylogenetic structure of whipworms in a multi-species community of non-human primates and humans in Western Uganda, using both traditional microscopy and molecular methods. A newly developed nested polymerase chain reaction (PCR) method aied to non-invasively collected fecal samples detected Trichuris with 100% sensitivity and 97% specificity relative to microscopy. Infection rates varied significantly among host species, from 13.3% in chimpanzees (Pan troglodytes) to 88.9% in olive baboons (Papio anubis). Phylogenetic analyses based on nucleotide sequences of the Trichuris internal transcribed spacer regions 1 and 2 of ribosomal DNA revealed three co-circulating Trichuris groups. Notably, one group was detected only in humans, while another infected all screened host species, indicating that whipworms from this group are transmitted among wild primates and humans. Conclusions and Significance:Our results suggest that the host range of Trichuris varies by taxonomic group, with some groups showing host specificity, and others showing host generality. In particular, one Trichuris taxon should be considered a multi-host pathogen that is capable of infecting wild primates and humans. This challenges past assumptions about the host specificity of this and similar helminth parasites and raises concerns about animal and human health. © 2014 Ghai et al.

Format EndNote

Vous pouvez importer cette référence dans EndNote.

Format BibTeX-CSV

Vous pouvez importer cette référence en format BibTeX-CSV.

Format BibTeX

Vous pouvez copier l'entrée BibTeX de cette référence ci-bas, ou l'importer directement dans un logiciel tel que JabRef .

@ARTICLE { GhaiSimonsChapmanEtAl2014,
    AUTHOR = { Ghai, R.R. and Simons, N.D. and Chapman, C.A. and Omeja, P.A. and Davies, T.J. and Ting, N. and Goldberg, T.L. },
    TITLE = { Hidden Population Structure and Cross-species Transmission of Whipworms (Trichuris sp.) in Humans and Non-human Primates in Uganda },
    JOURNAL = { PLoS Neglected Tropical Diseases },
    YEAR = { 2014 },
    VOLUME = { 8 },
    NUMBER = { 10 },
    NOTE = { cited By 1 },
    ABSTRACT = { Background:Whipworms (Trichuris sp.) are a globally distributed genus of parasitic helminths that infect a diversity of mammalian hosts. Molecular methods have successfully resolved porcine whipworm, Trichuris suis, from primate whipworm, T. trichiura. However, it remains unclear whether T. trichiura is a multi-host parasite capable of infecting a wide taxonomic breadth of primate hosts or a complex of host specific parasites that infect one or two closely related hosts. Methods and Findings:We examined the phylogenetic structure of whipworms in a multi-species community of non-human primates and humans in Western Uganda, using both traditional microscopy and molecular methods. A newly developed nested polymerase chain reaction (PCR) method aied to non-invasively collected fecal samples detected Trichuris with 100% sensitivity and 97% specificity relative to microscopy. Infection rates varied significantly among host species, from 13.3% in chimpanzees (Pan troglodytes) to 88.9% in olive baboons (Papio anubis). Phylogenetic analyses based on nucleotide sequences of the Trichuris internal transcribed spacer regions 1 and 2 of ribosomal DNA revealed three co-circulating Trichuris groups. Notably, one group was detected only in humans, while another infected all screened host species, indicating that whipworms from this group are transmitted among wild primates and humans. Conclusions and Significance:Our results suggest that the host range of Trichuris varies by taxonomic group, with some groups showing host specificity, and others showing host generality. In particular, one Trichuris taxon should be considered a multi-host pathogen that is capable of infecting wild primates and humans. This challenges past assumptions about the host specificity of this and similar helminth parasites and raises concerns about animal and human health. © 2014 Ghai et al. },
    DOCUMENT_TYPE = { Article },
    DOI = { 10.1371/journal.pntd.0003256 },
    KEYWORDS = { internal transcribed spacer; ribosome DNA, Article; controlled study; DNA extraction; DNA isolation; feces analysis; gene sequence; genetic variability; Haplorhini; host range; human; infection rate; microscopy; nonhuman; nucleotide sequence; nucleotide sequence; Pan troglodytes; Papio anubis; parasite identification; parasite prevalence; parasite transmission; phylogeny; polymerase chain reaction; population structure; primate; Romanomermis; sensitivity and specificity; taxonomy; trichuriasis; Trichuris; Trichuris muris; Trichuris suis; Trichuris trichiura; Trichuris vulpis; Uganda },
    PAGE_COUNT = { 9 },
    SOURCE = { Scopus },
    URL = { http://www.scopus.com/inward/record.url?eid=2-s2.0-84920527885&partnerID=40&md5=ee7df370b038f98e80a40b789b23551b },
}

********************************************************** ***************** Facebook Twitter *********************** **********************************************************

Abonnez-vous à
l'Infolettre du CEF!

********************************************************** ***************** Pub - Mycorhizes_2019 ****************** **********************************************************

********************************************************** ***************** Pub - Symphonies_Boreales ****************** **********************************************************

********************************************************** ***************** Boîte à trucs *************** **********************************************************

CEF-Référence
La référence vedette !

Jérémie Alluard (2016) Les statistiques au moments de la rédaction 

  • Ce document a pour but de guider les étudiants à intégrer de manière appropriée une analyse statistique dans leur rapport de recherche.

Voir les autres...