Alvarado-VillalobosCringoliMaurelliEtAl2017

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Alvarado-Villalobos, M.A., Cringoli, G., Maurelli, M.P., Cambou, A., Rinaldi, L., Barbachano-Guerrero, A., Guevara, R., Chapman, C.A., Serio-Silva, J.C. (2017) Flotation techniques (FLOTAC and mini-FLOTAC) for detecting gastrointestinal parasites in howler monkeys. Parasites and Vectors, 10(1). (Scopus )

Résumé

Background: Analyses of environmental correlates of the composition of gastrointestinal parasite communities in black howler monkeys (Alouatta pigra) have been hindered by inadequate calibration techniques of detection and quantification methods of the parasites. Here we calibrate samples and compare the likelihood of parasite detection using two flotation techniques, FLOTAC and Mini-FLOTAC, and compare flotation solution, preservation method and dilution ratio for egg detection and counts of the most common parasites (Controrchis spp. and Trypanoxyuris spp.) in howler monkeys. Results: For samples preserved in 5% formalin, the Mini-FLOTAC technique was the best option for qualitative and quantitative copro-microscopic analysis. This technique displays an 83.3% and 100% detection of Controrchis spp. and Trypanoxyuris spp. infections, respectively. For the trematode Controrchis spp., more eggs per gram of feces (EPG) were recorded with the flotation solution (FS) #7 (zinc sulfate; specific gravity SG = 1.35) at 1:20 and 1:25 dilution than other methods. By contrast, for the nematode Trypanoxyuris spp., the best results were recorded with FS1 (sucrose and formaldehyde; SG = 1.20) at 1:10 dilution. Conclusions: We recommend the Mini-FLOTAC technique for general use with parasite analysis on frugivore/folivores like the howler monkey, especially if many samples are analyzed. The technique has a high detection rate and the best EPG counts, allowing the qualitative and quantitative analysis of parasite load among the species or populations without the need for specialized equipment. © 2017 The Author(s).

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@ARTICLE { Alvarado-VillalobosCringoliMaurelliEtAl2017,
    AUTHOR = { Alvarado-Villalobos, M.A. and Cringoli, G. and Maurelli, M.P. and Cambou, A. and Rinaldi, L. and Barbachano-Guerrero, A. and Guevara, R. and Chapman, C.A. and Serio-Silva, J.C. },
    TITLE = { Flotation techniques (FLOTAC and mini-FLOTAC) for detecting gastrointestinal parasites in howler monkeys },
    JOURNAL = { Parasites and Vectors },
    YEAR = { 2017 },
    VOLUME = { 10 },
    NUMBER = { 1 },
    NOTE = { cited By 0 },
    ABSTRACT = { Background: Analyses of environmental correlates of the composition of gastrointestinal parasite communities in black howler monkeys (Alouatta pigra) have been hindered by inadequate calibration techniques of detection and quantification methods of the parasites. Here we calibrate samples and compare the likelihood of parasite detection using two flotation techniques, FLOTAC and Mini-FLOTAC, and compare flotation solution, preservation method and dilution ratio for egg detection and counts of the most common parasites (Controrchis spp. and Trypanoxyuris spp.) in howler monkeys. Results: For samples preserved in 5% formalin, the Mini-FLOTAC technique was the best option for qualitative and quantitative copro-microscopic analysis. This technique displays an 83.3% and 100% detection of Controrchis spp. and Trypanoxyuris spp. infections, respectively. For the trematode Controrchis spp., more eggs per gram of feces (EPG) were recorded with the flotation solution (FS) #7 (zinc sulfate; specific gravity SG = 1.35) at 1:20 and 1:25 dilution than other methods. By contrast, for the nematode Trypanoxyuris spp., the best results were recorded with FS1 (sucrose and formaldehyde; SG = 1.20) at 1:10 dilution. Conclusions: We recommend the Mini-FLOTAC technique for general use with parasite analysis on frugivore/folivores like the howler monkey, especially if many samples are analyzed. The technique has a high detection rate and the best EPG counts, allowing the qualitative and quantitative analysis of parasite load among the species or populations without the need for specialized equipment. © 2017 The Author(s). },
    AFFILIATION = { Red de Biología y Conservación de Vertebrados, Instituto de Ecología A.C, Xalapa Veracruz, Mexico; Unit of Parasitology and Parasitic Diseases, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy; ENSAIA (Ecole National e Supérieure d'Ágronomie et des Industries Alimentaires), Vandoeuvre-lés-Nancy, France; Laboratorio de Medicina de Conservación, Departamento de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, México, Mexico; Red de Biología Evolutiva, Instituto de Ecología A.C, Xalapa Veracruz, Mexico; Department of Anthropology and McGill School of Environment, McGill University, Montreal, QC, Canada },
    ART_NUMBER = { 586 },
    AUTHOR_KEYWORDS = { Alouatta; Gastrointestinal parasites; Howler monkeys; Nematodes; Quantitative flotation; Trematodes },
    DOCUMENT_TYPE = { Article },
    DOI = { 10.1186/s13071-017-2532-7 },
    SOURCE = { Scopus },
    URL = { https://www.scopus.com/inward/record.uri?eid=2-s2.0-85035310628&doi=10.1186%2fs13071-017-2532-7&partnerID=40&md5=452d2c8228bc94003fcc626afd922544 },
}

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